Boevink Petra, McLellan Hazel, Bukharova Tatyana, Engelhardt Stefan, Birch Paul
Cell and Molecular Sciences, The James Hutton Institute, Errol Road, Invergowrie, Dundee, DD2 5DA, UK,
Methods Mol Biol. 2014;1127:81-90. doi: 10.1007/978-1-62703-986-4_6.
Bimolecular fluorescence complementation (BiFC), performed with suitable controls and the right conditions, can be a straightforward and simple method to assess protein-protein interactions accessible to anyone with basic confocal microscopy skills. It is of course not without its own potential pitfalls and requires specific controls. Here we describe its use to study the interactions between pathogen effector proteins and host proteins inside plant cells.
在适当的对照和条件下进行的双分子荧光互补（BiFC），对于任何具备基本共聚焦显微镜操作技能的人来说，都可以是一种直接且简单的评估蛋白质 - 蛋白质相互作用的方法。当然，它也有自身潜在的缺陷，并且需要特定的对照。在这里，我们描述了其用于研究植物细胞内病原体效应蛋白与宿主蛋白之间相互作用的用途。
