Scicchitano R, Bienenstock J, Stanisz A M
Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
Brain Behav Immun. 1987 Sep;1(3):231-7. doi: 10.1016/0889-1591(87)90025-0.
The neuropeptides substance P (SP), somatostatin (SOM), and vasoactive intestinal peptide (VIP) have been shown to modulate lymphocyte DNA, RNA, and immunoglobulin synthesis. We have previously shown that SP enhances while SOM and VIP inhibit proliferation of murine splenic and Peyer's patch lymphocytes when cells were cultured with concanavalin A and neuropeptides for 72 h. Here we show that the effect of neuropeptides, in particular SP, is dependent on the amount of time that lymphocytes are incubated with NP. We found that SOM and VIP always inhibited cell proliferation for incubation times of 2 to 72 h. In contrast when cells were exposed to SP for 24 h or less, there was inhibition of [3H]thymidine uptake by both Peyer's patches and splenic lymphocytes. Significant enhancement in DNA synthesis by lymphocytes from both organs was only seen when cells were incubated with SP for the whole 72 h. We believe that our data may explain some of the conflicting reports regarding the effects of neuropeptides on cell proliferation.
神经肽P物质（SP）、生长抑素（SOM）和血管活性肠肽（VIP）已被证明可调节淋巴细胞的DNA、RNA和免疫球蛋白合成。我们之前已经表明，当细胞与伴刀豆球蛋白A和神经肽一起培养72小时时，SP可增强而SOM和VIP可抑制小鼠脾细胞和派伊尔结淋巴细胞的增殖。在这里我们表明，神经肽，特别是SP的作用，取决于淋巴细胞与神经肽孵育的时间。我们发现，SOM和VIP在2至72小时的孵育时间内总是抑制细胞增殖。相反，当细胞暴露于SP 24小时或更短时间时，派伊尔结和脾淋巴细胞对[3H]胸腺嘧啶核苷的摄取均受到抑制。只有当细胞与SP一起孵育整个72小时时，才能观察到来自两个器官的淋巴细胞的DNA合成有显著增强。我们认为，我们的数据可能解释了一些关于神经肽对细胞增殖影响的相互矛盾的报道。
