Scicchitano R, Biennenstock J, Stanisz A M
Department of Pathology and Intestinal Disease Research Unit, McMaster University, Hamilton, Ontario, Canada.
Immunology. 1988 Apr;63(4):733-5.
Many experiments have demonstrated that the nervous and immune systems interact in a bidirectional fashion. Neuropeptides, including substance P, have been shown to modulate lymphocyte DNA, RNA and immunoglobulin synthesis in vitro and to play a role in inflammatory and hypersensitivity disease states. However, the role of substance P as an immunomodulator in vivo is uncertain and there is only indirect evidence of this effect obtained in vitro. Therefore, we have assessed the effect of substance in vivo on DNA and immunoglobulin synthesis by murine splenic and Peyer's patch lymphocytes after the continuous administration via a miniosmotic pump of substance P in vivo. Substance P administered in this fashion increased cell proliferation of lymphocytes isolated from both organs. Immunoglobulin synthesis was also increased and in a relatively isotype-specific manner. IgA synthesis was most affected, IgM synthesis less so and IgG synthesis was not changed significantly. These effects of substance P on lymphocytes in vivo are similar to its effects on cell proliferation and immunoglobulin synthesis when cells are exposed to this peptide in vitro. These results provide direct evidence that neuropeptides (substance P) may modulate lymphocyte function in vivo and that neuropeptides should be incorporated into the conceptual framework of immune regulation.
许多实验表明，神经和免疫系统以双向方式相互作用。包括P物质在内的神经肽已被证明在体外可调节淋巴细胞的DNA、RNA和免疫球蛋白合成，并在炎症和超敏疾病状态中发挥作用。然而，P物质作为体内免疫调节剂的作用尚不确定，且仅有体外获得的这种效应的间接证据。因此，我们通过微型渗透泵在体内持续给药P物质后，评估了其对小鼠脾脏和派伊尔结淋巴细胞DNA和免疫球蛋白合成的体内效应。以这种方式给药的P物质增加了从两个器官分离的淋巴细胞的细胞增殖。免疫球蛋白合成也增加了，且以相对同型特异性的方式增加。IgA合成受影响最大，IgM合成次之，IgG合成无显著变化。P物质对体内淋巴细胞的这些作用与其在体外使细胞暴露于该肽时对细胞增殖和免疫球蛋白合成的作用相似。这些结果提供了直接证据，表明神经肽（P物质）可能在体内调节淋巴细胞功能，且神经肽应纳入免疫调节的概念框架。
