生长抑素对胰腺酶分泌的影响。

Effect of somatostatin on pancreatic enzyme secretion.

作者信息

Shinozaki H, Funakoshi A, Miyasaka K, Miyazaki K, Kitani K

机构信息

Third Department of Internal Medicine, Kyushu University Faculty of Medicine, Fukuoka, Japan.

出版信息

Gastroenterol Jpn. 1988 Dec;23(6):673-9. doi: 10.1007/BF02782954.

DOI:10.1007/BF02782954

PMID:2464524

Abstract

The effect of somatostatin (SS) on the pancreatic enzyme secretion was studied in a perfusion system using dispersed pancreatic rat acini in vitro. In addition the effect of SS on pancreatic secretion in vivo was also studied in conscious rats for comparison. In an in vitro study, 6 x 10(-7) M SS-14 caused no significant change in amylase release when added 20 min before stimulation by 10(-5) M carbamylcholine (Cch), 10(-6) M A23187, 5 x 10(-7) M secretin and 2 mM dibutyryl cyclic AMP. The addition of 6 x 10(-7) M SS-28 also caused no significant change in amylase release stimulated by 10(-5) M Cch. High performance liquid chromatographic examination indicated that no degradation of either SS-14 or SS-28 occurred after reaction with dispersed acini. In an in vivo study SS-14 caused marked inhibition of basal pancreatic secretion and stimulated pancreatic secretion by bile-pancreatic juice diversion. These results indicate that SS has no direct inhibitory action on rat pancreatic secretion, and that SS may inhibit the pancreatic secretion by indirect mechanisms.

摘要

在体外使用分散的大鼠胰腺腺泡的灌注系统中研究了生长抑素（SS）对胰腺酶分泌的影响。此外，还在清醒大鼠体内研究了SS对胰腺分泌的影响以作比较。在一项体外研究中，在由10⁻⁵M氨甲酰胆碱（Cch）、10⁻⁶M A23187、5×10⁻⁷M促胰液素和2mM二丁酰环磷腺苷刺激前20分钟添加6×10⁻⁷M SS - 14时，淀粉酶释放没有显著变化。添加6×10⁻⁷M SS - 28对由10⁻⁵M Cch刺激的淀粉酶释放也没有显著影响。高效液相色谱检查表明，与分散的腺泡反应后，SS - 14或SS - 28均未发生降解。在一项体内研究中，SS - 14对基础胰腺分泌有明显抑制作用，并通过胆胰液引流刺激胰腺分泌。这些结果表明，SS对大鼠胰腺分泌没有直接抑制作用，且SS可能通过间接机制抑制胰腺分泌。