Bernardini G, Belgiojoso P, Camatini M
Dipartimento di Biologia, Università di Milano, Italy.
Gamete Res. 1988 Dec;21(4):403-8. doi: 10.1002/mrd.1120210408.
The motility status of Xenopus laevis spermatozoa does not affect their respiration rate. Oxygen consumption for 10(9) spermatozoa is approximately 0.4 mumol/minute. Oxygen consumption is not increased by gramicidin D, an uncoupler, and it is not blocked by KCN or NaN3. The adenosine triphosphate (ATP) content of spermatozoa that have been activated is definitely less than that in the spermatozoa that remained immotile. Incubation in KCN, NaN3, and gramicidin decreases the ATP content and impairs motility. The conclusions of the present study are that in Xenopus spermatozoa motility and oxygen consumption are not correlated, and the composition of the respiratory chain of these spermatozoa presents noteworthy peculiarities.
非洲爪蟾精子的运动状态不影响其呼吸速率。10⁹个精子的耗氧量约为0.4微摩尔/分钟。解偶联剂短杆菌肽D不会增加耗氧量，且耗氧量不受氰化钾或叠氮化钠的抑制。已激活精子的三磷酸腺苷（ATP）含量肯定低于未运动精子的ATP含量。在氰化钾、叠氮化钠和短杆菌肽中孵育会降低ATP含量并损害运动能力。本研究的结论是，非洲爪蟾精子的运动和耗氧量不相关，且这些精子呼吸链的组成具有显著特点。
