Halangk W, Bohnensack R
Biomed Biochim Acta. 1986;45(3):331-41.
The turbidimetric method according to SOKOLOSKI et al. (Fertil. Steril. 28, 1337-1341, 1977) was used for quantitative measurement of the motility of washed bovine spermatozoa. The method is based on the registration of the increase in absorbance (A538) which is produced by spermatozoa entering the light path after run from the bottom of a photometer cuvette. This increase in absorbance is dependent on the amount of motile cells and their average velocity. The increase in absorbance related to the total cell concentration was found to be a highly reproducible quantity (motility index M). Inhibitors of dynein ATPase (vanadate) and of the mitochondrial ATP-production (KCN, carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP), oligomycin) produced a diminution of the motility index M in a concentration-dependent manner. Under conditions of oxidative energy metabolism (lactate as substrate) a linear relation between respiration and M existed. Such a relationship was found under treatment of spermatozoa with KCN or vanadate as well as for untreated sperm suspensions. The linear correlation to the respiratory rate justifies to use the proposed motility index as a relative measure of the average motility of a sperm sample.
采用索科洛夫斯基等人（《生育与不育》，第28卷，第1337 - 1341页，1977年）提出的比浊法对洗涤后的牛精子活力进行定量测定。该方法基于记录吸光度（A538）的增加，这是由精子从光度计比色皿底部游动进入光路所产生的。这种吸光度的增加取决于活动细胞的数量及其平均速度。与总细胞浓度相关的吸光度增加被发现是一个高度可重复的量（活力指数M）。动力蛋白ATP酶抑制剂（钒酸盐）以及线粒体ATP生成抑制剂（氰化钾、羰基氰对三氟甲氧基苯腙（FCCP）、寡霉素）以浓度依赖的方式使活力指数M降低。在氧化能量代谢条件下（以乳酸为底物），呼吸作用与M之间存在线性关系。在用氰化钾或钒酸盐处理精子以及未处理的精子悬液时都发现了这种关系。与呼吸速率的线性相关性证明将所提出的活力指数用作精子样本平均活力的相对测量指标是合理的。
