Cieśla Łukasz, Staszek Dorota, Kowalska Teresa, Waksmundzka-Hajnos Monika
J AOAC Int. 2013 Nov-Dec;96(6):1228-32. doi: 10.5740/jaoacint.sgeciesla.
TLC coupled with 2,2-diphenyl-1-picrylhydrazyl staining was used to analyze phenolic acid fractions of selected Salvia and Thymus species. Documented videoscans were processed by means of an image processing program. This is the first time that free phenolic acids fractions, as well as fractions containing phenolic acids derived from basic and acidic hydrolysis, have been analyzed and compared for selected sage and thyme species. The analyzed samples along with caffeic acid (CA; standard) were chromatographed on silica gel plates with toluene-ethyl acetate-formic acid (60 + 40 + 1, v/v/v) mobile phase. The extracts were investigated with respect to the activity of CA. It was found that CA was most abundant in the fractions derived from basic hydrolysis. This compound was not detected in any of the fractions obtained after acidic hydrolysis. S. officinalis and S. triloba have similar free radical scavenging activity fingerprints obtained for all the analyzed fractions.
采用薄层色谱法结合2,2-二苯基-1-苦基肼染色法分析了所选鼠尾草属和百里香属植物的酚酸组分。通过图像处理程序对记录的视频扫描进行处理。这是首次对所选鼠尾草和百里香属植物的游离酚酸组分以及通过碱性和酸性水解得到的含酚酸组分进行分析和比较。将分析样品与咖啡酸（CA；标准品）一起在硅胶板上进行色谱分离，流动相为甲苯-乙酸乙酯-甲酸（60 + 40 + 1，v/v/v）。对提取物进行了CA活性研究。结果发现，CA在碱性水解得到的组分中含量最高。在酸性水解后得到的任何组分中均未检测到该化合物。对于所有分析组分，药用鼠尾草和三叶鼠尾草具有相似的自由基清除活性指纹图谱。
