De Alwis Hemakanthi G, Girard Lauren, Kijak Philip J, Rummel Nathan
J AOAC Int. 2013 Nov-Dec;96(6):1233-8. doi: 10.5740/jaoacint.12-368.
A simple, robust LC-UV method was developed to assay erythromycin in medicated salmonid feed. In this method, erythromycin was extracted from feed with acetonitrile and water, cleaned up by SPE, evaporated to dryness, reconstituted, and analyzed by LC-UV. The resulting method produced high accuracy, 82-90%, for both salmon and trout feed that represented varied pellet sizes and ingredient amounts. The intraday and interday precisions, at < or = 6 and 5%, respectively, indicated the method's good repeatability. Calibration was linear over the range of drug concentrations typically used in medicated feed. An independent analyst validation further demonstrated the repeatability of the method. The developed method could support the drug approval process for erythromycin in medicated salmonid fish feed.
开发了一种简单、稳健的液相色谱-紫外法来测定药饵型鲑鱼饲料中的红霉素。在该方法中，用乙腈和水从饲料中提取红霉素，通过固相萃取进行净化，蒸发至干，复溶，然后用液相色谱-紫外法进行分析。所得方法对代表不同颗粒大小和成分含量的鲑鱼和鳟鱼饲料均具有82%-90%的高精度。日内和日间精密度分别≤6%和5%，表明该方法具有良好的重复性。在药饵型饲料通常使用的药物浓度范围内，校准呈线性。独立分析师验证进一步证明了该方法的重复性。所开发的方法可为药饵型鲑鱼饲料中红霉素的药物审批过程提供支持。
