Department of Chemistry Education, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, South Korea.
Nanoscale Res Lett. 2014 Mar 19;9(1):129. doi: 10.1186/1556-276X-9-129.
DNA-encapsulated silver nanodots are noteworthy candidates for bio-imaging probes, thanks to their excellent photophysical properties. The spectral shift of silver nanodot emitters from red to blue shows excellent correlations with the concentration of reactive oxygen species, which makes it possible to develop new types of probes for reactive oxygen species (ROS), such as hypochlorous acid (HOCl), given the outstanding stability of the blue in oxidizing environments. HOCl plays a role as a microbicide in immune systems but, on the other hand, is regarded as a disease contributor. Moreover, it is a common ingredient in household cleaners. There are still great demands to detect HOCl fluxes and their physiological pathways. We introduce a new ratiometric luminescence imaging method based on silver nanodots to sensitively detect hypochlorite. The factors that influence the accuracy of the detection are investigated. Its availability has also been demonstrated by detecting the active component in cleaners.
82; 82.30.Nr; 82.50.-m.
由于其出色的光物理特性，包裹在 DNA 中的银纳米点是生物成像探针的重要候选者。银纳米点发射器的光谱从红色到蓝色的位移与活性氧（ROS）的浓度显示出极好的相关性，这使得有可能开发新的活性氧（ROS）探针，例如次氯酸（HOCl），因为蓝色在氧化环境中具有出色的稳定性。HOCl 在免疫系统中作为杀菌剂发挥作用，但另一方面，它被认为是疾病的促成因素。此外，它也是家用清洁剂中的常见成分。仍然需要检测 HOCl 通量及其生理途径。我们引入了一种基于银纳米点的新比率荧光成像方法来灵敏地检测次氯酸盐。研究了影响检测准确性的因素。通过检测清洁剂中的活性成分也证明了其可用性。
82；82.30.Nr；82.50.-m。
