School of Chemistry and Biochemistry and Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, GA 30332-0400, USA.
Photochem Photobiol Sci. 2011 Jan;10(1):109-15. doi: 10.1039/c0pp00263a. Epub 2010 Nov 9.
Through tailored oligonucleotide scaffolds, Ag nanocluster syntheses have yielded thermally and cell-culture medium stable silver cluster-based emitters. Optimizing ssDNA stability has enabled creation of highly concentrated and spectrally pure nanocluster emitters with strong intracellular emission. Both fixed and live-cell staining become possible, and intracellular delivery is demonstrated both through conjugation to cell-penetrating peptides and via microinjection.
通过定制的寡核苷酸支架，已经合成了热稳定和细胞培养基稳定的基于银纳米簇的发射体。优化 ssDNA 的稳定性，使得能够创建高浓度和光谱纯的纳米簇发射体，具有强的细胞内发射。既可以进行固定细胞染色，也可以进行活细胞染色，并且通过与穿透肽缀合和微注射，已经证明了细胞内递药。
