Remy J J, Texier B, Chiocchia G, Charreire J
Institut National de la Santé et de la Recherche Médicale U. 283, Paris, France.
J Immunol. 1989 Feb 15;142(4):1129-33.
Clones of cytotoxic thyroid-specific T cell hybridomas were generated by fusion of thyroglobulin-primed, "in vitro"-boosted CBA lymph node cells with the AKR-derived lymphoma cell line BW 5147. One hundred and thirty one clones were obtained. Among them, 15 were able to induce the lysis of 51Cr-labeled syngeneic thyroid epithelial cells after 5 h of incubation at 37 degrees C. Two T cell clones, HTC1 and HTC2, were further studied. These clones, which exhibit cell surface characteristics of cytotoxic cells, were specific for only syngeneic thyroid cells (allogeneic thyroid cells or syngeneic epithelial cells were never lysed by these hybridomas). Moreover, by using Ag-pulsed syngeneic macrophages as targets, syngeneic cytotoxicity was shown to be specific for thyroglobulin and not for a nonrelated Ag. The lysis obtained with these autoreactive thyroid-specific T cell clones is restricted to class I major histocompatibility Ag. This property is assessed by both the blocking of syngeneic cytotoxicity toward thyroid epithelial cells or thyroglobulin-pulsed macrophages only by anti-class I mAb and by the detection of specific lysis of target cells exclusively when effector hybrid cells and target thyroid epithelial cells or thyroglobulin-pulsed macrophages shared at least class I major histocompatibility Ag.
通过将用甲状腺球蛋白致敏并在“体外”增强的CBA淋巴结细胞与源自AKR的淋巴瘤细胞系BW 5147融合，产生了细胞毒性甲状腺特异性T细胞杂交瘤克隆。共获得131个克隆。其中，15个在37℃孵育5小时后能够诱导对51Cr标记的同基因甲状腺上皮细胞的裂解。对两个T细胞克隆HTC1和HTC2进行了进一步研究。这些表现出细胞毒性细胞表面特征的克隆仅对同基因甲状腺细胞具有特异性（这些杂交瘤从未裂解异基因甲状腺细胞或同基因上皮细胞）。此外，通过使用用抗原脉冲处理的同基因巨噬细胞作为靶细胞，显示同基因细胞毒性对甲状腺球蛋白具有特异性，而对无关抗原则无特异性。用这些自身反应性甲状腺特异性T细胞克隆获得的裂解作用仅限于I类主要组织相容性抗原。这一特性通过以下两种方式来评估：仅用抗I类单克隆抗体阻断对甲状腺上皮细胞或甲状腺球蛋白脉冲巨噬细胞的同基因细胞毒性，以及仅当效应杂交细胞与靶甲状腺上皮细胞或甲状腺球蛋白脉冲巨噬细胞至少共享I类主要组织相容性抗原时检测靶细胞的特异性裂解。
