Remy J J, Salamero J, Charreire J
Mol Biol Med. 1986 Apr;3(2):167-79.
We observed T lymphoblast generation after three days of culture of normal spleen lymphocytes on monolayers of syngeneic thyroid epithelial cells. It appears that only these T lymphoblasts sensitized on thyroid monolayers are specifically labelled by fluorescein-conjugated thyroglobulin. In this study, the role of thyroglobulin, and the manner in which it is presented to syngeneic T cells bearing receptors for thyroglobulin were investigated. It appears that thyroglobulin plays a key role in primary syngeneic sensitization of spleen cells in vitro, but its action is not exclusive. Proteins, which are encoded by a gene located in the I-A subregion level of the major histocompatibility complex, are capable of inducing a primary proliferative signal when the presentation of syngeneic class II antigens by syngeneic thyroid epithelial cells is simultaneous. In addition, thyroglobulin-pulsed macrophages are not able to do so. In contrast, once this primary syngeneic T cell proliferation has been accomplished, soluble thyroglobulin is sufficient to induce a secondary response by these syngeneic T cells.
我们观察到，正常脾淋巴细胞在同基因甲状腺上皮细胞单层上培养三天后会产生T淋巴母细胞。似乎只有在甲状腺单层上致敏的这些T淋巴母细胞会被荧光素偶联的甲状腺球蛋白特异性标记。在本研究中，我们研究了甲状腺球蛋白的作用，以及它呈递给带有甲状腺球蛋白受体的同基因T细胞的方式。甲状腺球蛋白似乎在体外脾细胞的初次同基因致敏中起关键作用，但它的作用并非唯一的。当同基因甲状腺上皮细胞同时呈递同基因II类抗原时，由位于主要组织相容性复合体I-A亚区水平的基因编码的蛋白质能够诱导初次增殖信号。此外，用甲状腺球蛋白脉冲处理的巨噬细胞则无法做到这一点。相反，一旦这种初次同基因T细胞增殖完成，可溶性甲状腺球蛋白就足以诱导这些同基因T细胞产生二次反应。
