Klinman D M, Morse H C
Infectious Diseases Branch, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD 20892.
J Immunol. 1989 Feb 15;142(4):1144-9.
A syndrome characterized by lymphadenopathy, hypergammaglobulinemia, and immunodeficiency develops in C57BL/6 mice inoculated with LP-BM5 murine leukemia viruses. By studying the number and antigenic specificity of B cells activated in the course of this disease, we found that a series of reproducible changes in the humoral immune system were induced by retroviral infection. The rate of B cell proliferation and the proportion of B cells activated to secrete Ig increased by nearly 10-fold at 4 wk post inoculation. B cells producing antibodies reactive with a panel of three conventional Ag and five autoantigens were stimulated simultaneously and proportionally to secrete, demonstrating that such activation was polyclonal in nature. At 12 wk post infection, the number of Ig-secreting B cells continued to rise and significant hypergammaglobulinemia developed. At 16 wk post infection, immunostimulation gave way to immunosuppression, as evidenced by a slight decline in the number of Ig-secreting lymphocytes and a sharp reduction in the concentration of serum antibody. At this time, the B cell repertoires of infected mice diverged markedly from those of uninfected animals. These changes are comparable to those found in some patients infected with HIV, and provide a useful model to study the association between retroviral infection and regulatory abnormalities of the humoral immune system.
接种LP - BM5鼠白血病病毒的C57BL/6小鼠会出现一种以淋巴结病、高球蛋白血症和免疫缺陷为特征的综合征。通过研究在这种疾病过程中被激活的B细胞的数量和抗原特异性，我们发现逆转录病毒感染诱导了体液免疫系统一系列可重复的变化。接种后4周，B细胞增殖率和被激活分泌Ig的B细胞比例增加了近10倍。产生与一组三种传统抗原和五种自身抗原反应的抗体的B细胞同时受到刺激并按比例分泌，表明这种激活本质上是多克隆的。感染后12周，分泌Ig的B细胞数量持续上升，出现显著的高球蛋白血症。感染后16周，免疫刺激让位于免疫抑制，表现为分泌Ig的淋巴细胞数量略有下降和血清抗体浓度急剧降低。此时，感染小鼠的B细胞库与未感染动物的B细胞库明显不同。这些变化与一些感染HIV的患者中发现的变化相似，并为研究逆转录病毒感染与体液免疫系统调节异常之间的关联提供了一个有用的模型。
