Kirsebom L A, Baer M F, Altman S
Department of Biology, Yale University, New Haven, CT 06520.
J Mol Biol. 1988 Dec 20;204(4):879-88. doi: 10.1016/0022-2836(88)90048-4.
We have studied the efficiency of suppression by tRNA suppressors in vivo in strains of Escherichia coli that harbor a mutation in the rnpA gene, the gene for the protein component (C5) of RNase P, and in strains that carry several different alleles of the rnpB gene, the gene for the RNA component (M1) of RNase P. Depending on the genetic background, different efficiencies of suppression by the various tRNA suppressors were observed. Thus, mutations in rnpA have separable and distinct effects from mutations in rnpB on the processing of tRNA precursors by RNase P. In addition, the efficiency of suppression by several derivatives of E. coli tRNA(Tyr) Su3 changed as the genetic background was altered.
我们研究了tRNA抑制子在体内对大肠杆菌菌株的抑制效率，这些菌株中rnpA基因（核糖核酸酶P的蛋白质组分（C5）的基因）发生了突变，以及携带rnpB基因（核糖核酸酶P的RNA组分（M1）的基因）几种不同等位基因的菌株。根据遗传背景，观察到各种tRNA抑制子的抑制效率不同。因此，rnpA中的突变与rnpB中的突变对核糖核酸酶P加工tRNA前体具有可分离且不同的影响。此外，随着遗传背景的改变，大肠杆菌tRNA(Tyr) Su3的几种衍生物的抑制效率也发生了变化。
