鼠冠状病毒保守中和表位的氨基酸序列。
Amino acid sequence of a conserved neutralizing epitope of murine coronaviruses.
作者信息
Luytjes W, Geerts D, Posthumus W, Meloen R, Spaan W
机构信息
Institute of Virology, Veterinary Faculty, University of Utrecht, The Netherlands.
出版信息
J Virol. 1989 Mar;63(3):1408-12. doi: 10.1128/JVI.63.3.1408-1412.1989.
We identified the binding site of monoclonal antibody 19.2, which cross-neutralizes several mouse hepatitis virus (MHV) strains, inhibits fusion of MHV-infected cells, and protects against lethal infection (P. J. Talbot and M. J. Buchmeier, Virus Res. 2:317-328, 1985). We used fusion proteins, generated by expression of fragments of the MHV A59 E2 gene in pEX plasmids, and synthetic peptides in a PEPSCAN.
我们确定了单克隆抗体19.2的结合位点，该抗体可交叉中和多种小鼠肝炎病毒（MHV）毒株，抑制MHV感染细胞的融合，并预防致死性感染（P. J. 塔尔博特和M. J. 布赫迈尔，《病毒研究》2:317 - 328，1985年）。我们使用了通过在pEX质粒中表达MHV A59 E2基因片段产生的融合蛋白，以及在PEPSCAN中使用的合成肽。
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