Collins A R, Knobler R L, Powell H, Buchmeier M J
Virology. 1982 Jun;119(2):358-71. doi: 10.1016/0042-6822(82)90095-2.
Hybridoma cell lines producing monoclonal antibodies to the JHM strain of mouse hepatitis virus-4 (MHV-4) were established. By indirect immunofluorescence and immune precipitation, monoclonal antibodies of three viral polypeptide specificities were characterized. Monoclonal antibodies to nucleocapsid reacted in the cytoplasm of infected cells and precipitated the 60,000d nucleocapsid polypeptide (VP-4) of MHV-4. Other monoclonal antibodies reacted both in the cytoplasm and on the surface of infected cells and were found to precipitate the 170,000d viral glycoprotein (GP-1). A third set of monoclonal antibodies reacted both in the cytoplasm and on the surface of infected cells and precipitated the 25,000d viral glycoprotein (GP-5) and its precursor VP-6 (23,000d). AntiGP-1 alone had direct neutralizing activity for MHV-4 virus, while in the presence of complement both anti-GP-1 and anti-GP-5 neutralized virus. Only anti-GP-1 had the ability to inhibit the spread of infection due to fusion in L241 cells. Thus, the viral glycoprotein GP-1 likely contains both the attachment and fusion activities of MHV-4.
建立了产生针对小鼠肝炎病毒4型（MHV-4）JHM株单克隆抗体的杂交瘤细胞系。通过间接免疫荧光和免疫沉淀，对三种病毒多肽特异性的单克隆抗体进行了表征。针对核衣壳的单克隆抗体在感染细胞的细胞质中发生反应，并沉淀出MHV-4的60,000d核衣壳多肽（VP-4）。其他单克隆抗体在感染细胞的细胞质和表面均发生反应，并被发现沉淀出170,000d病毒糖蛋白（GP-1）。第三组单克隆抗体在感染细胞的细胞质和表面均发生反应，并沉淀出25,000d病毒糖蛋白（GP-5）及其前体VP-6（23,000d）。单独的抗GP-1对MHV-4病毒具有直接中和活性，而在补体存在的情况下，抗GP-1和抗GP-5均能中和病毒。只有抗GP-1具有抑制L241细胞中由于融合导致的感染传播的能力。因此，病毒糖蛋白GP-1可能同时包含MHV-4的附着和融合活性。
