Center for Drug Safety Evaluation and Research, Shanghai University of Traditional Chinese Medicine.
Biosci Trends. 2014 Feb;8(1):32-7. doi: 10.5582/bst.8.32.
The present study is designed to search for the serum cytokine biomarker for liver injury induced by Dioscorea bulbifera L., which is a traditionally used herbal medicine in China. Mice were orally given various doses of ethyl acetate extract (EF) isolated from D. bulbifera for 12 days. The activity of serum alanine/aspartate transaminases (ALT/AST) was increased in EF (400 mg/kg)-treated mice. Histological assessment further confirmed EF (400 mg/kg)-induced liver injury. Results of a cytokine-antibody array demonstrated that there were 10 cytokines up-regulated and 1 cytokine down-regulated in EF (400 mg/kg)-treated mice. Enzyme-linked immunosorbent assay (ELISA) further confirmed the increased level of CD30 ligand (CD30L) and decreased level of interlukin-3 (IL-3) in EF-treated mice. In conclusion, our results demonstrate that the altered expression of CD30L and IL-3 may be potential biomarkers for hepatotoxicity induced by D. bulbifera.
本研究旨在寻找薯蓣（中国传统药用植物）所致肝损伤的血清细胞因子生物标志物。将薯蓣的乙酸乙酯提取物（EF）以不同剂量给小鼠口服给药 12 天。EF（400mg/kg）处理的小鼠血清丙氨酸/天冬氨酸转氨酶（ALT/AST）活性升高。组织学评估进一步证实 EF（400mg/kg）诱导的肝损伤。细胞因子抗体阵列的结果表明，EF（400mg/kg）处理的小鼠中有 10 种细胞因子上调和 1 种细胞因子下调。酶联免疫吸附试验（ELISA）进一步证实 EF 处理的小鼠中 CD30 配体（CD30L）水平升高和白细胞介素-3（IL-3）水平降低。总之，我们的结果表明，CD30L 和 IL-3 的表达改变可能是薯蓣所致肝毒性的潜在生物标志物。
