Matsumura Y, Nakase K, Ikegawa R, Hayashi K, Ohyama T, Morimoto S
Department of Pharmacology, Osaka University of Pharmaceutical Sciences, Japan.
Life Sci. 1989;44(2):149-57. doi: 10.1016/0024-3205(89)90533-x.
The effect of endothelin, a newly identified endothelium-derived vasoconstrictor peptide, on renin release from rat kidney cortical slices was examined. Endothelin produced a concentration-dependent inhibition of renin release and this inhibitory effect was dependent on extracellular calcium. The dihydropyridine calcium channel blockers nifedipine and nicardipine did not antagonize the inhibitory effect induced by endothelin. On the other hand, nifedipine completely antagonized the extracellular high potassium- or Bay K 8644-induced inhibition of renin release. The endothelin-induced inhibition of the release was markedly blocked by the addition of Co2+. Similar blocking effects of Co2+ were also observed with extracellular high potassium or Bay K 8644. Thus, endothelin exerts an inhibitory action on renin release in vitro, in a calcium-dependent manner. This inhibition may be mediated by the increased calcium influx through dihydropyridine-insensitive calcium channels.
研究了新发现的内皮源血管收缩肽内皮素对大鼠肾皮质切片肾素释放的影响。内皮素对肾素释放产生浓度依赖性抑制，且这种抑制作用依赖于细胞外钙。二氢吡啶类钙通道阻滞剂硝苯地平和尼卡地平不能拮抗内皮素诱导的抑制作用。另一方面，硝苯地平完全拮抗细胞外高钾或Bay K 8644诱导的肾素释放抑制。加入Co2+可显著阻断内皮素诱导的释放抑制。细胞外高钾或Bay K 8644也观察到Co2+的类似阻断作用。因此，内皮素在体外以钙依赖方式对肾素释放发挥抑制作用。这种抑制可能是由通过对二氢吡啶不敏感的钙通道增加的钙内流介导的。
