Sato Y, Wake K, Watanabe I
Department of Otolaryngology, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
Acta Otolaryngol Suppl. 1988;454:48-52. doi: 10.3109/00016488809125004.
By the immunohistochemical method using anti-keratin antibodies (KL1-antigen, 56 KD keratin; PKK1-antigen, 40-52.5 KD keratin) and electron microscopy, the changes in cell shapes and the cytokeratin components of the epithelial cells during the infiltration of lymphocytes were studied. In the surface epithelium, PKK1 reacts with only the keratinocytes in the basal layer, while KL1 stains in the spinous layer. In the neck portion of the crypt, transformed keratinocytes scattered in the spinous layer react intensely with PKK1. These cells issue prolonged cytoplasmic processes which surround the cavities filled with infiltrating lymphocytes. In the deep portion of the crypt, PKK1-positive cells interconnect with one another constituting the network of the star-shaped reticular cells. The enlarged intercellular spaces of the reticular cells are filled with many lymphocytes. Our observations suggest that the infiltration of lymphocytes into the crypt epithelium induces the change in keratin expression of the epithelial cells in the human palatine tonsils, and the change of keratin molecules makes the stratified flattened epithelium to transform into the network of star-shaped reticular cells.
运用抗角蛋白抗体（KL1抗原，56KD角蛋白；PKK1抗原，40 - 52.5KD角蛋白）的免疫组织化学方法以及电子显微镜技术，研究了淋巴细胞浸润过程中上皮细胞的细胞形态变化和细胞角蛋白成分。在表面上皮中，PKK1仅与基底层的角质形成细胞发生反应，而KL1则在棘层染色。在隐窝颈部，散在于棘层的转化角质形成细胞与PKK1发生强烈反应。这些细胞发出延长的细胞质突起，围绕着充满浸润淋巴细胞的腔隙。在隐窝深部，PKK1阳性细胞相互连接，构成星形网状细胞网络。网状细胞扩大的细胞间隙中充满了许多淋巴细胞。我们的观察结果表明，淋巴细胞浸润到隐窝上皮会诱导人腭扁桃体上皮细胞角蛋白表达的变化，角蛋白分子的变化使复层扁平上皮转化为星形网状细胞网络。
