Choi G, Suh Y L, Lee H M, Jung K Y, Hwang S J
Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, Korea.
Acta Otolaryngol Suppl. 1996;523:28-33.
The tonsillar crypt shows infiltration of lymphoid cells in the epithelium and the highly porous or discontinuous basement membrane. However, the relationship between developmental period and the significance of the disrupted epithelial basement membrane and infiltration of lymphoid cells within the human tonsillar crypt epithelium is still obscure. The present study was designed to determine the prenatal and postnatal changes of the human tonsillar crypt epithelium in 4 fetuses, 4 neonates, 1 infant, 5 children and 4 adults using immunohistochemical stains with polyclonal anti-laminin and monoclonal anti-type IV collagen antibody, and transmission electron microscope. The tonsillar crypt epithelium from all fetuses and neonates showed infiltration of many lymphoid cells and an intact basement membrane by immunohistochemistry. Transmission electron microscope revealed pores in the basement membrane through which lymphocytes passed. Tonsils from a 4-month-old infant showed focal disruption of the basement membrane by immunohistochemical staining. The tonsils from children and adults showed that there was massive disruption of the basement membrane with lymphoid cell infiltration in the tonsillar crypt epithelium by immunohistochemical staining and transmission electron microscopy. In conclusion, lymphoid cell infiltration and holes of the basement membrane of the tonsillar crypt epithelium are normal developmental findings, whereas disruption of the basement membrane of the tonsillar crypt epithelium is pathological.
扁桃体隐窝显示上皮内有淋巴细胞浸润，基底膜高度多孔或不连续。然而，人类扁桃体隐窝上皮内发育时期与上皮基底膜破坏及淋巴细胞浸润的意义之间的关系仍不清楚。本研究旨在通过使用多克隆抗层粘连蛋白和单克隆抗IV型胶原抗体的免疫组织化学染色以及透射电子显微镜，确定4例胎儿、4例新生儿、1例婴儿、5例儿童和4例成人的人类扁桃体隐窝上皮的产前和产后变化。通过免疫组织化学，所有胎儿和新生儿的扁桃体隐窝上皮均显示有许多淋巴细胞浸润且基底膜完整。透射电子显微镜显示基底膜有孔隙，淋巴细胞可通过这些孔隙。4个月大婴儿的扁桃体通过免疫组织化学染色显示基底膜有局灶性破坏。通过免疫组织化学染色和透射电子显微镜检查，儿童和成人的扁桃体显示扁桃体隐窝上皮内基底膜有大量破坏且伴有淋巴细胞浸润。总之，扁桃体隐窝上皮的淋巴细胞浸润和基底膜孔是正常的发育表现，而扁桃体隐窝上皮基底膜的破坏是病理性的。
