一种在薄层聚丙烯酰胺凝胶等电聚焦后对大鼠脑内硫胺素焦磷酸酶和其他磷酸酶进行染色的显色转换方法。

A lead conversion method for staining thiamine pyrophosphatase and other phosphatases in rat brain after thin layer polyacrylamide gel isoelectric focusing.

作者信息

Mazzarello P, Poloni M, Patrini C, Bosone D, Rindi G

机构信息

Clinica Neurologica I, Università di Milano, Italy.

出版信息

Basic Appl Histochem. 1988;32(4):495-500.

PMID:2464990

Abstract

Rat brain thiamine pyrophosphatase (TPPase) was separated by thin layer polyacrylamide gel isoelectric focusing (IF) and stained with a modification of the lead conversion method of Allen and Hyncik (1963). 10 bands with TPPase activity were observed in the pH range 4.6-7.1. The overall IF pattern of TPPase was similar to that of uridine diphosphatase and inosine diphosphatase but was clearly different from that of adenosine diphosphatase, p-nitrophenyl phosphatase, alpha-naphthyl phosphatase and thiamine monophosphatase. A semiquantitative assessment of TPPase isoenzymes has been performed using laser densitometry.

摘要

大鼠脑硫胺素焦磷酸酶（TPPase）通过薄层聚丙烯酰胺凝胶等电聚焦（IF）进行分离，并用对Allen和Hyncik（1963年）铅转化方法的改良方法进行染色。在pH范围4.6 - 7.1内观察到10条具有TPPase活性的条带。TPPase的整体IF模式与尿苷二磷酸酶和肌苷二磷酸酶相似，但与腺苷二磷酸酶、对硝基苯磷酸酶、α-萘基磷酸酶和硫胺素单磷酸酶明显不同。已使用激光密度测定法对TPPase同工酶进行了半定量评估。