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兔角膜内皮中核苷二磷酸酶和硫胺素焦磷酸酶活性的细胞化学定位

Cytochemical localiztion of nucleoside diphosphatase and thiamine pyrophosphatase activities in the rabbit corneal endothelium.

作者信息

Yokota S, Waller W K

出版信息

Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1977 Mar 29;202(1):45-54. doi: 10.1007/BF00496767.

DOI:10.1007/BF00496767
PMID:193413
Abstract

Localization of NDPase and TPPase activities in the rabbit corneal endothelium was investigated by cytochemical methods. After incubation at both pH 7.0 and 8.0, the NDPase activity was detected in cisternae of ER, nuclear envelope, and Golgi saccules, while the TPPase activity was demonstrated only in Golgi saccules. The reaction products of both enzymes were decreased when incubated at pH 8.0. The NDPase activity was inhibited by uranyl nitrate but not by sodium fluoride, while the TPPase was inhibited by fluoride but not by uranium.

摘要

采用细胞化学方法研究了兔角膜内皮中核苷二磷酸酶(NDPase)和焦磷酸酶(TPPase)活性的定位。在pH 7.0和8.0条件下孵育后,在内质网池、核膜和高尔基体囊泡中检测到NDPase活性,而TPPase活性仅在高尔基体囊泡中显示。在pH 8.0条件下孵育时,两种酶的反应产物均减少。NDPase活性受硝酸铀酰抑制,但不受氟化钠抑制,而TPPase受氟化物抑制,但不受铀抑制。

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