Laforenza U, Patrini C, Mazzarello P, Poloni M, Rindi G
Institute of Human Physiology, University of Pavia, Italy.
Basic Appl Histochem. 1990;34(4):249-57.
Thiamine and thiamine mono-, pyro- and triphosphate were found at detectable levels in synaptosomes isolated from whole rat brain. Synaptosomes prepared from whole brain, cerebellum and medulla were also found to contain uridine and inosine mono- and diphosphatases as well as the thiamine pyrophosphate synthetizing and hydrolyzing enzymes, but no thiamine monophosphatase. By isoelectric focusing on thin layer polyacrylamide gel of Triton X-100 homogenates of synaptosomes, thiamine pyrophosphatase activity could be separated into 10 bands with different isoelectric points. The contents of thiamine compounds and enzymes in synaptosomes were generally lower than those found in neuronal cell bodies.
在从全鼠脑分离出的突触体中发现了可检测水平的硫胺素及其一磷酸、焦磷酸和三磷酸形式。还发现，从全脑、小脑和延髓制备的突触体含有尿苷和肌苷单磷酸酶及二磷酸酶，以及硫胺素焦磷酸合成酶和水解酶，但不含硫胺素单磷酸酶。通过对突触体的Triton X-100匀浆在薄层聚丙烯酰胺凝胶上进行等电聚焦，硫胺素焦磷酸酶活性可被分离成10条具有不同等电点的条带。突触体中硫胺素化合物和酶的含量通常低于神经元细胞体中的含量。
