Svanholm H, Nielsen B, Starklint H
Department of Pathology, University Hospital of Odense, Denmark.
APMIS Suppl. 1988;4:100-8.
Keratin patterns in benign prostatic hyperplasia and prostatic adenocarcinoma were evaluated using frozen and formalin-fixed tissue. Five different commercially available antibodies were used. In frozen tissue basal cells of benign acinic and ductal structures stained with PKE, and to a lesser degree with MCA 144, CAM 5.2 and RCK 102. Luminal cells stained with MCA 144, CAM 5.2, RCK 102 and to a lesser degree with PKE. In formalin-fixed tissue basal cells stained exclusively with Z622, predominantly with PKE and RCK 102, and to lesser degree with MCA 144 and CAM 5.2. Luminal cells stained with MCA 144 and CAM 5.2 and to some degree with PKE and RCK 102, while Z622 stained luminal cells of ductal epithelium weakly, but acinic cells not at all. Luminal phenotype dominated in prostatic adenocarcinomas which were stained with MCA 144 and CAM 5.2 irrespective of differentiation, and independently of whether frozen or formalin-fixed tissue was used. However, the different keratin phenotype of benign and malignant prostatic epithelium depends to some degree on the immunohistochemical procedures used. In diagnostic pathology Z622 may be able to separate intraluminal neoplastic lesions from invasive carcinoma, a problem particularly seen when cribriform growth pattern is found.
使用冷冻组织和福尔马林固定组织评估良性前列腺增生和前列腺腺癌中的角蛋白模式。使用了五种不同的市售抗体。在冷冻组织中，良性腺泡和导管结构的基底细胞用PKE染色，用MCA 144、CAM 5.2和RCK 102染色的程度较轻。腔面细胞用MCA 144、CAM 5.2、RCK 102染色，用PKE染色的程度较轻。在福尔马林固定组织中，基底细胞仅用Z622染色，主要用PKE和RCK 102染色，用MCA 144和CAM 5.2染色的程度较轻。腔面细胞用MCA 144和CAM 5.2染色，在一定程度上用PKE和RCK 102染色，而Z622对导管上皮的腔面细胞染色较弱，但对腺泡细胞完全不染色。无论分化程度如何，也无论使用冷冻组织还是福尔马林固定组织，前列腺腺癌中均以腔面表型为主，用MCA 144和CAM 5.2染色。然而，前列腺良性和恶性上皮细胞不同的角蛋白表型在一定程度上取决于所使用的免疫组织化学方法。在诊断病理学中，Z622可能能够将腔内肿瘤性病变与浸润性癌区分开来，这一问题在发现筛状生长模式时尤为常见。
