Brawer M K, Peehl D M, Stamey T A, Bostwick D G
Cancer Res. 1985 Aug;45(8):3663-7.
Keratin immunoreactivity in the benign and neoplastic human prostate was examined immunohistochemically using two monoclonal antibodies with differing specificities. One of these antibodies stained only the basal cells of the normal and hyperplastic prostatic epithelium, with no reactivity in tumor cells of prostatic adenocarcinoma. The other monoclonal antibody recognized a keratin protein present in all normal and hyperplastic columnar (secretory) epithelial cells, as well as in all cancer cells regardless of degree of tumor differentiation. In addition, the second antibody stained acinar and ductal epithelial cells exhibiting premalignant changes. Our findings indicate that keratin immunoreactivity differs among the epithelial cell populations of the human prostate, probably reflecting expression of different keratin proteins. The distinctive patterns of staining obtained with these two antibodies may assist in distinguishing hyperplastic from neoplastic prostatic epithelium, as well as in the recognition of basal cell hyperplasia, transitional cell metaplasia, and premalignant changes.
使用两种具有不同特异性的单克隆抗体，通过免疫组织化学方法检测了良性和肿瘤性人类前列腺中的角蛋白免疫反应性。其中一种抗体仅对正常和增生性前列腺上皮的基底细胞染色，对前列腺腺癌的肿瘤细胞无反应。另一种单克隆抗体识别存在于所有正常和增生性柱状（分泌）上皮细胞以及所有癌细胞中的一种角蛋白，而与肿瘤分化程度无关。此外，第二种抗体对表现出癌前变化的腺泡和导管上皮细胞染色。我们的研究结果表明，人类前列腺上皮细胞群体中的角蛋白免疫反应性不同，这可能反映了不同角蛋白的表达。用这两种抗体获得的独特染色模式可能有助于区分增生性前列腺上皮和肿瘤性前列腺上皮，以及识别基底细胞增生、移行细胞化生和癌前变化。
