Zhong Cheng, Sun Zhuo, Zhou Zhao, Jin Ming-Jie, Tan Zhi-Lei, Jia Shi-Ru
Key Laboratory of Industrial Fermentation Microbiology (Ministry of Education), School of Biotechnology, Tianjin University of Science and Technology , Tianjin 300457, People's Republic of China.
Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University , P.O. Box 6888, Tianjin 300072, People's Republic of China.
J Agric Food Chem. 2014 Apr 9;62(14):3217-3222. doi: 10.1021/jf500349s. Epub 2014 Apr 1.
Plant-based proteins are valuable supplements to compensate the gap between supply and demand in the food or feed industry. However, they lack essential amino acids, such as lysine in cereal grains and sulfur-containing amino acids in legumes, which greatly limit their wider uses for human and animals. In this study, the contents of nutritional ingredients and antinutritional factors of Caragana korshinskii Kom. and its protein isolates were quantitatively investigated. It was shown that the crude protein contents of C. korshinskii Kom. and its protein isolates obtained by alkaline extraction method (Al-CPI) and acetone precipitation method (A-CPI) were 9.1, 50.1, and 42.6%, respectively. Amino acid contents in C. korshinskii Kom., Al-CPI, and A-CPI basically exceeded the FAO/WHO (2007) reference pattern for adults except sulfur-containing amino acids. The lysine levels in C. korshinskii Kom., Al-CPI, and A-CPI were 4.1, 3.1, and 3.8 mg/100 mg crude protein respectively, which were higher than some other kinds of cereal grains. The methionine in A-CPI (1.39 mg/100 mg crude protein) was even higher than that in soybean. The antinutritional factors in C. korshinskii Kom. and Al-CPI were generally lower than those in some other kinds of legumes except total phenol and tannin. Total phenol and tannins in Al-CPI were 19.02 and 5.66 mg/g dry substance, respectively, but they were undetectable in A-CPI. This study provided a detailed analysis on nutritional and antinutritional factors in C. korshinskii Kom. and its protein isolates, indicating that they have a great potential on food and feed additives.
植物性蛋白质是弥补食品或饲料行业供需缺口的宝贵补充剂。然而，它们缺乏必需氨基酸，如谷物中的赖氨酸和豆类中的含硫氨基酸，这极大地限制了它们在人类和动物中的更广泛应用。在本研究中，对柠条锦鸡儿及其分离蛋白的营养成分和抗营养因子含量进行了定量研究。结果表明，柠条锦鸡儿及其通过碱提取法（Al-CPI）和丙酮沉淀法（A-CPI）获得的分离蛋白的粗蛋白含量分别为9.1%、50.1%和42.6%。柠条锦鸡儿、Al-CPI和A-CPI中的氨基酸含量除含硫氨基酸外，基本超过了粮农组织/世界卫生组织（2007年）成人参考模式。柠条锦鸡儿、Al-CPI和A-CPI中的赖氨酸水平分别为4.1、3.1和3.8mg/100mg粗蛋白，高于其他一些谷物。A-CPI中的蛋氨酸（1.39mg/100mg粗蛋白）甚至高于大豆中的蛋氨酸。柠条锦鸡儿和Al-CPI中的抗营养因子一般低于其他一些豆类，但总酚和单宁除外。Al-CPI中的总酚和单宁分别为19.02和5.66mg/g干物质，但在A-CPI中未检测到。本研究对柠条锦鸡儿及其分离蛋白中的营养和抗营养因子进行了详细分析，表明它们在食品和饲料添加剂方面具有巨大潜力。
