Zhou Wenna, Xu Heng, Luan Guangxiang, Wang HongLun, Wang Xiaoyan, Ji Mingkai
Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.
Skin Pharmacol Physiol. 2016;29(5):243-249. doi: 10.1159/000448739. Epub 2016 Sep 16.
The objective of this study was to evaluate the effects of seed oil of Caragana korshinskii Kom. against Trichophyton mentagrophytes on an in vivo guinea pig model of dermatophytosis.
The skin of albino guinea pigs was infected with T. mentagrophytes, and the animals were divided into five groups: negative control (NC group), positive control (PC group), vehicle control, CK50% group (received topical 50% seed oil of C.korshinskii), and CK100% group (received topical 100% seed oil of C.korshinskii). Evaluation of clinical efficacy was performed 72 h after the completion of a 10-day treatment regimen. Skin biopsy samples were processed for histopathological examination.
The infected untreated control guinea pigs showed patches of hair loss and ulcerated or scaly skin. Lower clinical scores indicate improved efficacy compared with NC. The lesion scores significantly declined in the CK50%, CK100%, and PC groups in comparison with the NC group. The CK50% group (45.31%) and the CK100% group (75%) showed clinical efficacy compared with the PC group (78.13%). In addition, no fungal elements, inflammation, or tissue destruction was observed in any of the PAS-stained sections of the infected skin in the groups treated with CK100% or 1% terbinafine.
Seed oil of C.korshinskii demonstrated high antifungal efficacy in experimental dermatophytosis.
本研究的目的是在皮肤癣菌病的体内豚鼠模型上评估柠条锦鸡儿种子油对须癣毛癣菌的作用。
将白化豚鼠的皮肤感染须癣毛癣菌，动物被分为五组：阴性对照（NC组）、阳性对照（PC组）、赋形剂对照、CK50%组（接受局部涂抹50%柠条锦鸡儿种子油）和CK100%组（接受局部涂抹100%柠条锦鸡儿种子油）。在完成10天治疗方案后72小时进行临床疗效评估。对皮肤活检样本进行组织病理学检查。
未治疗的感染对照豚鼠出现脱发斑以及皮肤溃疡或鳞屑。较低的临床评分表明与NC组相比疗效有所改善。与NC组相比，CK50%组、CK100%组和PC组的病变评分显著下降。与PC组（78.13%）相比，CK50%组（45.31%）和CK100%组（75%）显示出临床疗效。此外，在接受CK100%或1%特比萘芬治疗的组中，感染皮肤的任何PAS染色切片均未观察到真菌成分、炎症或组织破坏。
柠条锦鸡儿种子油在实验性皮肤癣菌病中显示出高抗真菌疗效。
