Department of Genetics, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Department of Genetics, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Methods. 2014 Aug 1;68(3):487-91. doi: 10.1016/j.ymeth.2014.03.008. Epub 2014 Mar 17.
Carbon dioxide (CO2) is an important molecule in cell metabolism. It is also a byproduct of many physiological processes. In humans, impaired lung function and lung diseases disrupt the body's ability to dispose of CO2 and elevate its levels in the body (hypercapnia). Animal models allow further understanding of how CO2 is sensed in the body and what are the physiological responses to high CO2 levels. This information can provide new strategies in the battle against the detrimental effects of CO2 accumulation in lung diseases. The nematode Caenorhabditis elegans provides us with such a model animal due to its natural ability to sense and navigate through varying concentrations of CO2, as well as the fact that it can be genetically manipulated with ease. Here we describe the different methods used to measure the effects elevated levels of CO2 have on the molecular sensing mechanism and physiology of C. elegans.
二氧化碳（CO2）是细胞代谢中的重要分子。它也是许多生理过程的副产物。在人类中，受损的肺功能和肺部疾病会破坏身体清除 CO2 的能力，并导致体内 CO2 水平升高（高碳酸血症）。动物模型可以进一步了解 CO2 在体内是如何被感知的，以及对高 CO2 水平的生理反应是什么。这些信息可以为对抗肺部疾病中 CO2 积累的有害影响提供新的策略。秀丽隐杆线虫为我们提供了这样的模式生物，因为它具有天然感知和在不同浓度的 CO2 中导航的能力，而且它可以很容易地进行基因操作。在这里，我们描述了不同的方法来测量升高的 CO2 水平对秀丽隐杆线虫分子感知机制和生理学的影响。
