Department of Chemistry, University of South Florida, Tampa, FL 33620, USA.
Department of Chemistry, University of South Florida, Tampa, FL 33620, USA.
FEBS Lett. 2014 May 2;588(9):1596-602. doi: 10.1016/j.febslet.2014.02.051. Epub 2014 Mar 17.
Long-chain fatty acid amides are cell-signaling lipids identified in mammals and, recently, in invertebrates, as well. Many details regarding fatty acid amide metabolism remain unclear. Herein, we demonstrate that Drosophila melanogaster is an excellent model system for the study long-chain fatty acid amide metabolism as we have quantified the endogenous levels of N-acylglycines, N-acyldopamines, N-acylethanolamines, and primary fatty acid amides by LC/QTOF-MS. Growth of D. melanogaster on media supplemented with [1-(13)C]-palmitate lead to a family of (13)C-palmitate-labeled fatty acid amides in the fly heads. The [1-(13)C]-palmitate feeding studies provide insight into the biosynthesis of the fatty acid amides.
长链脂肪酸酰胺是在哺乳动物中发现的细胞信号脂质，最近也在无脊椎动物中发现。许多关于脂肪酸酰胺代谢的细节仍然不清楚。在此，我们证明黑腹果蝇是研究长链脂肪酸酰胺代谢的极好模型系统，因为我们已经通过 LC/QTOF-MS 定量了内源性 N-酰基甘氨酸、N-酰基多巴胺、N-酰基乙醇胺和初级脂肪酸酰胺的水平。在补充 [1-(13)C]-棕榈酸的培养基中生长的黑腹果蝇的头部中出现了一系列 (13)C-棕榈酸标记的脂肪酸酰胺。[1-(13)C]-棕榈酸的喂养研究为脂肪酸酰胺的生物合成提供了深入的了解。
