Sharif A, Goussault Y
INSERM U-180 Faculté de Médicine, Paris, France.
Cancer Biochem Biophys. 1988 Jul;10(1):17-24.
We have investigated the effect of bleomycin (BLM) on thymidine phosphorylation in lectin-stimulated normal human lymphocytes. BLM reduces thymidine phosphorylation by decreasing the activity of thymidine kinase (TK). Accordingly, polyacrylamide gel electrophoresis (PAGE) of extracts of cells incubated for 48, 72 and 96 h showed here that this activity dropped 48, 65 and 67% respectively. The electrophoretic profiles of TK activity were similar but different in amplitude. These effects of the BLM were confirmed firstly by direct measurement of TK activity, secondly by amount of 3H-thymidine incorporation in the cultures before cell lysis. Both the measurement of TK activity and 3H-thymidine incorporation were correlated.
我们研究了博来霉素（BLM）对凝集素刺激的正常人淋巴细胞中胸苷磷酸化的影响。博来霉素通过降低胸苷激酶（TK）的活性来减少胸苷磷酸化。因此，在此处对培养48、72和96小时的细胞提取物进行的聚丙烯酰胺凝胶电泳（PAGE）显示，该活性分别下降了48%、65%和67%。TK活性的电泳图谱相似但幅度不同。博来霉素的这些作用首先通过直接测量TK活性得到证实，其次通过细胞裂解前培养物中3H-胸苷的掺入量得到证实。TK活性的测量和3H-胸苷的掺入量均具有相关性。
