Shortman K, Wilson A, Pearse M, Gallagher P, Scollay R
Walter and Eliza Hall Institute for Medical Research, Parkville, Vic., Australia.
Immunol Cell Biol. 1988 Oct-Dec;66 ( Pt 5-6):423-33. doi: 10.1038/icb.1988.54.
'Double negative' (CD4-CD8-) thymocytes from adult mice of different inbred strains were examined for surface expression of CD3 and of various forms of the T cell antigen receptor (TcR), as well as for the levels of subpopulations defined by the surface markers HSA ('heat stable antigen', recognized by M1/69, J11d and B2A2), CD5 (Ly 1) and Thy 1. Marked variations were found in the level of the double negative subsets which were surface TcR+, or which were HSA-CD5+; these generally varied together since most CD4-CD8-HSA-CD5+ thymocytes were TCR+. The level of the CD3-TCR complex on the surface of those double negative thymocytes which were TcR+ was as high as on mature T cells in some strains (CBA/Ca), but was much lower in other strains (C57BL/6J). In most mouse strains the CD4-CD8-HSA-CD5+ thymocytes expressed predominantly the alpha beta form of the TcR, with an exceptionally high (70%) usage of V beta 8 gene products. In strains which lacked V beta 8 expressing T cells due to a deletion of the V beta 8 gene region, reduced levels of alpha beta TcR+ cells were found within the CD4-CD8- thymocytes; the HSA-CD5+ subset was then only present at low levels (as in SJL/J and C57BR mice) or was present at a high level but expressed predominantly gamma delta TcR (as in SWR mice). The results suggest that the accumulation of CD4-CD8-TcR+ HSA-CD5+ thymocytes is a selective event, and that their developmental pathway is off the mainstream of T cell maturation in the thymus.
对来自不同近交系成年小鼠的“双阴性”（CD4-CD8-）胸腺细胞进行检测，以分析其CD3及各种形式的T细胞抗原受体（TcR）的表面表达情况，以及由表面标志物HSA（“热稳定抗原”，可被M1/69、J11d和B2A2识别）、CD5（Ly 1）和Thy 1所定义的亚群水平。结果发现，表面TcR+或HSA-CD5+的双阴性亚群水平存在显著差异；由于大多数CD4-CD8-HSA-CD5+胸腺细胞为TCR+，所以这些差异通常是共同变化的。在某些品系（CBA/Ca）中，TcR+的双阴性胸腺细胞表面的CD3-TCR复合物水平与成熟T细胞上的水平一样高，但在其他品系（C57BL/6J）中则要低得多。在大多数小鼠品系中，CD4-CD8-HSA-CD5+胸腺细胞主要表达TcR的αβ形式，Vβ8基因产物的使用率异常高（70%）。在由于Vβ8基因区域缺失而缺乏表达Vβ8的T细胞的品系中，CD4-CD8-胸腺细胞内αβTcR+细胞水平降低；此时，HSA-CD5+亚群仅以低水平存在（如在SJL/J和C57BR小鼠中），或以高水平存在但主要表达γδTcR（如在SWR小鼠中）。结果表明，CD4-CD8-TcR+HSA-CD5+胸腺细胞的积累是一个选择性事件，并且它们的发育途径偏离了胸腺中T细胞成熟的主流途径。
