Mohr Stephanie E, Hu Yanhui, Kim Kevin, Housden Benjamin E, Perrimon Norbert
Drosophila RNAi Screening Center, Harvard Medical School, Boston, Massachusetts 02115.
Genetics. 2014 May;197(1):1-18. doi: 10.1534/genetics.113.154344. Epub 2014 Mar 20.
Drosophila melanogaster has become a system of choice for functional genomic studies. Many resources, including online databases and software tools, are now available to support design or identification of relevant fly stocks and reagents or analysis and mining of existing functional genomic, transcriptomic, proteomic, etc. datasets. These include large community collections of fly stocks and plasmid clones, "meta" information sites like FlyBase and FlyMine, and an increasing number of more specialized reagents, databases, and online tools. Here, we introduce key resources useful to plan large-scale functional genomics studies in Drosophila and to analyze, integrate, and mine the results of those studies in ways that facilitate identification of highest-confidence results and generation of new hypotheses. We also discuss ways in which existing resources can be used and might be improved and suggest a few areas of future development that would further support large- and small-scale studies in Drosophila and facilitate use of Drosophila information by the research community more generally.
黑腹果蝇已成为功能基因组学研究的首选系统。现在有许多资源，包括在线数据库和软件工具，可用于支持相关果蝇品系和试剂的设计或鉴定，以及对现有功能基因组学、转录组学、蛋白质组学等数据集的分析和挖掘。这些资源包括果蝇品系和质粒克隆的大型社区集合、像FlyBase和FlyMine这样的“元”信息网站，以及越来越多更专业的试剂、数据库和在线工具。在这里，我们介绍了一些关键资源，这些资源对于规划果蝇大规模功能基因组学研究以及以有助于识别最高可信度结果和产生新假设的方式分析、整合和挖掘这些研究结果很有用。我们还讨论了现有资源的使用方式和可能的改进方法，并提出了一些未来发展领域，这些领域将进一步支持果蝇的大规模和小规模研究，并更广泛地促进研究社区对果蝇信息的使用。
