蟾酥乙醇提取物体外抗骨肉瘤活性研究
Study on anti-osteosarcoma activity of ethanol extract of Venenum bufonis in vitro.
作者信息
Chen Guangxing, Jiang Xuefeng, Li Jingyi, Duan Guangjie, Yang Liu, Zhang Ying, Wang Fuyou
机构信息
Center for Joint Surgery, Southwest Hospital, Chongqing, 400038, China.
Institute of Pathology and Southwest Cancer Center, Third Military Medical University, Chongqing, 400038, China.
出版信息
Afr J Tradit Complement Altern Med. 2013 Nov 2;11(1):73-7. eCollection 2014.
BACKGROUND
Venenum bufonis is the dried white secretion of the auricular and skin glands of Bufo gargarizans Cantor, or Bufo melanostictus Schneider, Bufonidae. It is used in the treatment of deep-rooted carbuncle, boils and swelling; pain in the throat, heart stroke, coma, abdominal pain, vomiting and diarrhea. The objective of this paper is to preliminarily observe the effects of ethanol extract of Venenum bufonis on growth, and proliferation of human osteosarcoma U2OS cell lines, and to provide a theoretical basis for an in-depth study of the clinical application of Venenum bufonis for osteosarcoma inhibition, with its mechanism of action.
MATERIALS AND METHODS
SRB assay was used to determine the effect of Venenum bufonis ethanol extract on U2OS cell line activity, and to detect its inhibitory dose-effect on osteosarcoma cells. FCM was applied to determine the effect of Venenum bufonis ethanol extract on U2OS cell apoptosis and to perform cell cycle analysis.
RESULTS
As results, different Venenum bufonis ethanol extracts showed apparent concentration-effect relationships on U2OS cell lines. FCM analysis showed that it had a U2OS apoptosis promoting effect, which increased with increasing concentration. Cell cycle analysis revealed that the Venenum bufonis ethanol extract mainly arrested U2OS in the G0/G1 phase, preventing the cells from progressing to the S phase.
CONCLUSION
The study concluded that Venenum bufonis ethanol extract has an inhibitory effect on proliferation of osteosarcoma U2OS cells.
背景
蟾酥是蟾蜍科中华大蟾蜍或黑眶蟾蜍耳后腺及皮肤腺的干燥白色分泌物。其用于治疗疔疮痈疽、咽喉肿痛、中暑神昏、腹痛吐泻等。本文旨在初步观察蟾酥乙醇提取物对人骨肉瘤U2OS细胞系生长及增殖的影响，为深入研究蟾酥抑制骨肉瘤的临床应用及其作用机制提供理论依据。
材料与方法
采用SRB法检测蟾酥乙醇提取物对U2OS细胞系活性的影响，检测其对骨肉瘤细胞的抑制剂量效应。应用流式细胞术（FCM）检测蟾酥乙醇提取物对U2OS细胞凋亡的影响并进行细胞周期分析。
结果
不同浓度的蟾酥乙醇提取物对U2OS细胞系呈现明显的浓度效应关系。FCM分析表明其具有促进U2OS细胞凋亡的作用，且随浓度增加而增强。细胞周期分析显示蟾酥乙醇提取物主要使U2OS细胞阻滞于G0/G1期，阻止细胞进入S期。
结论
本研究得出结论，蟾酥乙醇提取物对骨肉瘤U2OS细胞的增殖具有抑制作用。
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