使用细胞增殖测定法和活细胞成像技术评估蜂王浆作为细胞培养中胎牛血清替代品的效果。
Evaluation of royal jelly as an alternative to fetal bovine serum in cell culture using cell proliferation assays and live cell imaging.
作者信息
Musa Marahaini, Nasir Nurul Fatihah Mohamad, Thirumulu Kannan Ponnuraj
机构信息
School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia ; Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
出版信息
Afr J Tradit Complement Altern Med. 2013 Nov 2;11(1):148-55. eCollection 2014.
BACKGROUND
Royal jelly is a nutritious substance produced by the young nurse bees and contains significant amounts of proteins which are important for cell growth and proliferation. The aim of this study was to evaluate the effect of royal jelly as an alternative to fetal bovine serum (FBS) in cell culture using cell proliferation assays and live cell imaging.
MATERIALS AND METHODS
MRC-5 cells were treated with various concentrations of royal jelly extract in MTT assay. The control groups were comprised of Alpha-Minimal Essential Medium (α-MEM) alone and α-MEM with 10% FBS. Subsequently, the cell proliferation was studied for 10 days using Alamar Blue assay and live cell imaging from 48 to 72 h. The population doubling time (PDT) was determined using trypan blue assay after live cell imaging.
RESULTS
In MTT assay, 0.156 and 0.078 mg/ml of royal jelly produced higher cell viability compared to positive control group but were not significantly different (P > 0.05). In the Alamar Blue assay, 0.156 and 0.078 mg/ml of royal jelly produced greater percentage of reduction at day 3 even though no significant difference was found (P > 0.05). Based on live cell imaging, the PDT for positive, negative, 0.156 and 0.078 mg/ml of royal jelly groups were 29.09, 62.50, 41.67 and 41.67 h respectively. No significant difference was found in the PDT between all the groups (P > 0.05).
CONCLUSION
Royal jelly does not exhibit similar ability like FBS to facilitate cell growth under the present test conditions.
背景
蜂王浆是年轻保育蜂分泌的一种营养物质，含有大量对细胞生长和增殖很重要的蛋白质。本研究的目的是通过细胞增殖试验和活细胞成像，评估蜂王浆作为胎牛血清（FBS）替代品在细胞培养中的效果。
材料与方法
在MTT试验中，用不同浓度的蜂王浆提取物处理MRC - 5细胞。对照组分别为单独的α - 最低限度基本培养基（α - MEM）和含10% FBS的α - MEM。随后，使用Alamar Blue试验研究细胞增殖10天，并在48至72小时进行活细胞成像。活细胞成像后，用台盼蓝试验测定群体倍增时间（PDT）。
结果
在MTT试验中，0.156和0.078 mg/ml的蜂王浆与阳性对照组相比，细胞活力更高，但差异不显著（P > 0.05）。在Alamar Blue试验中，0.156和0.078 mg/ml的蜂王浆在第3天产生的还原百分比更高，尽管未发现显著差异（P > 0.05）。根据活细胞成像，阳性、阴性、0.156和0.078 mg/ml蜂王浆组的PDT分别为29.09、62.50、41.67和41.67小时。所有组之间的PDT没有显著差异（P > 0.05）。
结论
在当前测试条件下，蜂王浆促进细胞生长的能力不如FBS。
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