College of Biosystems Engineering and Food Science, Zhejiang University / Key Laboratory of Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs / Zhejiang Key Laboratory for Agri-Food Processing, Hangzhou 310058, China.
USDA ARS Nutritional Genomics Laboratory, JM-USDA Human Nutrition Research Center on Aging at Tufts University, Boston, Massachusetts 02111, the United States.
J Zhejiang Univ Sci B. 2018;19(12):960-972. doi: 10.1631/jzus.B1800257.
Royal jelly (RJ) from honeybee has been widely used as a health promotion supplement. The major royal jelly proteins (MRJPs) have been identified as the functional component of RJ. However, the question of whether MRJPs have anti-senescence activity for human cells remains. Human embryonic lung fibroblast (HFL-I) cells were cultured in media containing no MRJPs (A), MRJPs at 0.1 mg/ml (B), 0.2 mg/ml (C), or 0.3 mg/ml (D), or bovine serum albumin (BSA) at 0.2 mg/ml (E). The mean population doubling levels of cells in media B, C, D, and E were increased by 12.4%, 31.2%, 24.0%, and 10.4%, respectively, compared with that in medium A. The cells in medium C also exhibited the highest relative proliferation activity, the lowest senescence, and the longest telomeres. Moreover, MRJPs up-regulated the expression of superoxide dismutase-1 (SOD1) and down-regulated the expression of mammalian target of rapamycin (MTOR), catenin beta like-1 (CTNNB1), and tumor protein p53 (TP53). Raman spectra analysis showed that there were two unique bands related to DNA synthesis materials, amide carbonyl group vibrations and aromatic hydrogens. These results suggest that MRJPs possess anti-senescence activity for the HFL-I cell line, and provide new knowledge illustrating the molecular mechanism of MRJPs as anti-senescence factors.
蜂王浆（RJ）来自蜜蜂，已被广泛用作促进健康的补品。主要蜂王浆蛋白（MRJPs）已被确定为 RJ 的功能成分。然而，MRJPs 是否对人类细胞具有抗衰老活性仍存在疑问。人胚肺成纤维细胞（HFL-I）在不含 MRJPs 的培养基中（A）、含 0.1mg/mlMRJPs 的培养基中（B）、0.2mg/mlMRJPs 的培养基中（C）或 0.3mg/mlMRJPs 的培养基中（D）或含 0.2mg/ml 牛血清白蛋白（BSA）的培养基中（E）进行培养。与培养基 A 相比，培养基 B、C、D 和 E 中的细胞群体倍增水平分别提高了 12.4%、31.2%、24.0%和 10.4%。培养基 C 中的细胞还表现出最高的相对增殖活性、最低的衰老和最长的端粒。此外，MRJPs 上调了超氧化物歧化酶-1（SOD1）的表达，下调了哺乳动物雷帕霉素靶蛋白（MTOR）、连环蛋白β样 1（CTNNB1）和肿瘤蛋白 p53（TP53）的表达。拉曼光谱分析表明，有两个与 DNA 合成物质相关的独特带，酰胺羰基振动和芳氢。这些结果表明，MRJPs 对 HFL-I 细胞系具有抗衰老活性，并提供了新的知识，阐明了 MRJPs 作为抗衰老因子的分子机制。
