苦参化学成分的分离及其抗胶质瘤作用的研究。
A study on isolation of chemical constituents from Sophora flavescens Ait. and their anti-glioma effects.
作者信息
Zheng Kebin, Li Chunhui, Shan Xiaosong, Liu Haipeng, Fan Wufang, Wang Zhenshan
机构信息
College of Life Science, Hebei University, Baoding 071000, Hebei Province, China.
Department of Neurosurgery, Affiliated Hospital of Hebei University, Baoding 071000, Hebei Province, China.
出版信息
Afr J Tradit Complement Altern Med. 2013 Nov 2;11(1):156-60. doi: 10.4314/ajtcam.v11i1.24. eCollection 2014.
BACKGROUND
Sophora flavescens Ait. is a traditional Chinese medicine with a long history in China. It is mainly used in the treatment of heat dysentery and similar ailments in the clinical. The objective of this paper was to isolate, purify and identify alkaloids from Sophora flavescens Ait. and to explore their inhibitory effects on C6 glioma cells.
MATERIALS AND METHODS
Column chromatography, extraction and NMR spectroscopy were used to structurally identify the isolated compounds. MTT assay and flow cytometry were used to detect the inhibitory effect of matrine on C6 cells.
RESULTS
Three compounds were isolated from Sophora flavescens Ait., namely matrine, oxymatrine and lupeol. Different concentrations of matrine solution all had inhibitory effects on growth of C6 cell lines, which showed apparent dose-effect relationship. Compared with the control group, proportion of G0/G1 phase cells increased in each matrine concentration group to a maximum of 79.8%; proportion of S phase cells reduced, and proportion of G2/M phase cells declined slightly to a minimum of 6.3%, suggesting that after the action of matrine proliferation of C6 cells was significantly inhibited and the cells were arrested in the G1 phase.
CONCLUSION
We concluded that Sophora flavescens Ait. has an inhibitory effect on C6 cell proliferation.
背景
苦参是一种在中国有着悠久历史的传统中药。在临床上主要用于治疗热痢等类似病症。本文的目的是从苦参中分离、纯化和鉴定生物碱，并探讨它们对C6胶质瘤细胞的抑制作用。
材料与方法
采用柱色谱法、萃取法和核磁共振光谱法对分离得到的化合物进行结构鉴定。采用MTT法和流式细胞术检测苦参碱对C6细胞的抑制作用。
结果
从苦参中分离得到3种化合物，即苦参碱、氧化苦参碱和羽扇豆醇。不同浓度的苦参碱溶液对C6细胞系的生长均有抑制作用，呈明显的剂量效应关系。与对照组相比，各苦参碱浓度组G0/G1期细胞比例增加，最高达79.8%；S期细胞比例降低，G2/M期细胞比例略有下降，最低至6.3%，提示苦参碱作用后C6细胞增殖明显受到抑制，细胞阻滞于G1期。
结论
我们得出结论，苦参对C6细胞增殖有抑制作用。
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