苦豆子总生物碱对骨肉瘤细胞生长抑制作用的研究
Study on the inhibitory effect of total alkaliods of Sophora alopecuroides on osteosarcoma cell growth.
作者信息
Lu Xiang, Lin Bin, Tang Jia-guang, Cao Zheng, Hu Yuan
机构信息
Institute of Orthopedics, The First Affiliated Hospital of the General Hospital of PLA, Beijing 100048, People's Republic of China.
出版信息
Afr J Tradit Complement Altern Med. 2013 Nov 2;11(1):172-5. eCollection 2014.
BACKGROUND
Ku Dou Zi is the dried whole plant, roots and seeds of Sophora alopecuroides L. in the genus Sophora of family Leguminosae. The entire plant is bitter in taste, cold in nature, and has the heat clearing, detoxifying, pathogenic wind dispelling dampness, analgesic, and insecticidal effects. Modern pharmacological studies have proved that TASA has pharmacological activities of anti-cancer. The objective of this paper was to investigate the inhibitory effect of total alkaloids of Sophora alopecuroides (TASA), on osteosarcoma cell growth and its mechanism.
MATERIALS AND METHODS
MTT assay and flow cytometry were used to study the inhibitory effect of TASA on human osteosarcoma cell line OS732.
RESULTS
The results showed that the inhibition rates of different concentrations of TASA (1.5, 3, and 4.5g/kg), against human osteosarcoma cell line OS732, were: 18.4%, 27.4% and 52.8%, respectively.
CONCLUSION
TASA has an inhibitory effect on osteosarcoma cell growth.
背景
苦豆子为豆科槐属植物苦豆子的干燥全草、根及种子。全草味苦，性寒，具有清热、解毒、祛风除湿、止痛和杀虫的功效。现代药理学研究证明，苦豆子总生物碱具有抗癌药理活性。本文旨在研究苦豆子总生物碱（TASA）对骨肉瘤细胞生长的抑制作用及其机制。
材料与方法
采用MTT法和流式细胞术研究TASA对人骨肉瘤细胞系OS732的抑制作用。
结果
结果表明，不同浓度（1.5、3和4.5g/kg）的TASA对人骨肉瘤细胞系OS732的抑制率分别为18.4%、27.4%和52.8%。
结论
TASA对骨肉瘤细胞生长具有抑制作用。
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