Garcia-Gil M, Berton F, Tongiorgi E, Brunelli M
Department of Physiology and Biochemistry, University of Pisa, Italy.
J Neurochem. 1989 Mar;52(3):699-704. doi: 10.1111/j.1471-4159.1989.tb02511.x.
Protein phosphorylation plays an important role in the regulation of neural functions. We have studied the phosphorylation of proteins in homogenates of segmental ganglia of the leech Hirudo medicinalis. We describe a number of proteins whose phosphorylation is dependent on calcium/calmodulin or cyclic nucleotides. Most of the proteins whose phosphorylation is increased in the presence of calcium seem to be substrates for cyclic nucleotide-dependent protein kinases. Only two of the phosphoproteins described appear to be specific substrates for calcium/calmodulin protein kinase(s), and at least six phosphoproteins appear to be specific substrates for cyclic nucleotide-dependent kinase(s). The leech nervous system, with large and identifiable neurons, provides a good tool for studies of neural functions, such as learning. The results are discussed in the context of the role of protein phosphorylation on learning processes.
蛋白质磷酸化在神经功能调节中起着重要作用。我们研究了医用水蛭节段神经节匀浆中蛋白质的磷酸化情况。我们描述了许多其磷酸化依赖于钙/钙调蛋白或环核苷酸的蛋白质。在钙存在下磷酸化增加的大多数蛋白质似乎是环核苷酸依赖性蛋白激酶的底物。所描述的磷酸蛋白中只有两种似乎是钙/钙调蛋白蛋白激酶的特异性底物，并且至少有六种磷酸蛋白似乎是环核苷酸依赖性激酶的特异性底物。水蛭神经系统具有大且可识别的神经元，为研究诸如学习等神经功能提供了一个良好的工具。本文在蛋白质磷酸化对学习过程的作用背景下讨论了这些结果。
