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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Garcia-Gil M, Bottai D, Zaccardi M L, Cannizzaro M, Brunelli M

Department of Physiology and Biochemistry, University of Pisa, Italy.

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1993 Jan;104(1):125-31. doi: 10.1016/0742-8413(93)90123-3.

Phosphoproteins of different regions of the Hirudo medicinalis central nervous system have been analysed by means of two-dimensional electrophoresis. 2. Serotonin, 8-Br-cAMP and phorbol 12,13-dibutyrate stimulate phosphorylation of a number of proteins whose isoelectric points and molecular weights are presented. 3. A group of proteins of 78 kDa and pI = 6-6.5, whose level of phosphorylation increases in the presence of serotonin, 8-Br-cAMP and phorbol ester, is observed only in segmental but not in cephalic or caudal ganglia. 4. The putative roles of these phosphoproteins are discussed.

已通过二维电泳分析了医蛭中枢神经系统不同区域的磷蛋白。2. 血清素、8-溴环磷酸腺苷和佛波醇12,13-二丁酸酯刺激了一些蛋白质的磷酸化，文中给出了这些蛋白质的等电点和分子量。3. 观察到一组分子量为78 kDa、等电点为6 - 6.5的蛋白质，其磷酸化水平在血清素、8-溴环磷酸腺苷和佛波酯存在时会增加，且仅在节段神经节中出现，而在头部或尾部神经节中未出现。4. 讨论了这些磷蛋白的假定作用。

Garcia-Gil M, Bottai D, Zaccardi M L, Cannizzaro M, Brunelli M

Department of Physiology and Biochemistry, University of Pisa, Italy.

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1993 Jan;104(1):125-31. doi: 10.1016/0742-8413(93)90123-3.

Phosphoproteins of different regions of the Hirudo medicinalis central nervous system have been analysed by means of two-dimensional electrophoresis. 2. Serotonin, 8-Br-cAMP and phorbol 12,13-dibutyrate stimulate phosphorylation of a number of proteins whose isoelectric points and molecular weights are presented. 3. A group of proteins of 78 kDa and pI = 6-6.5, whose level of phosphorylation increases in the presence of serotonin, 8-Br-cAMP and phorbol ester, is observed only in segmental but not in cephalic or caudal ganglia. 4. The putative roles of these phosphoproteins are discussed.

已通过二维电泳分析了医蛭中枢神经系统不同区域的磷蛋白。2. 血清素、8-溴环磷酸腺苷和佛波醇12,13-二丁酸酯刺激了一些蛋白质的磷酸化，文中给出了这些蛋白质的等电点和分子量。3. 观察到一组分子量为78 kDa、等电点为6 - 6.5的蛋白质，其磷酸化水平在血清素、8-溴环磷酸腺苷和佛波酯存在时会增加，且仅在节段神经节中出现，而在头部或尾部神经节中未出现。4. 讨论了这些磷蛋白的假定作用。