Reddehase M J, Rothbard J B, Koszinowski U H
Federal Research Centre for Virus Diseases of Animals, Tübingen, FRG.
Nature. 1989 Feb 16;337(6208):651-3. doi: 10.1038/337651a0.
Peptides that are antigenic for T lymphocytes are ligands for two receptors, the class I or II glycoproteins that are encoded by genes in the major histocompatibility complex, and the idiotypic alpha/beta chain T-cell antigen receptor. That a peptide must bind to an MHC molecule to interact with a T-cell antigen receptor is the molecular basis of the MHC restriction of antigen-recognition by T lymphocytes. In such a trimolecular interaction the amino-acid sequence of the peptide must specify the contact with both receptors: agretope residues bind to the MHC receptor and epitope residues bind to the T-cell antigen receptor. From a compilation of known antigenic peptides, two algorithms have been proposed to predict antigenic sites in proteins. One algorithm uses linear motifs in the sequence, whereas the other considers peptide conformation and predicts antigenicity for amphipathic alpha-helices. We report here that a systematic delimitation of an antigenic site precisely identifies a predicted pentapeptide motif as the minimal antigenic determinant presented by a class I MHC molecule and recognized by a cytolytic T lymphocyte clone.
对T淋巴细胞具有抗原性的肽是两种受体的配体，即主要组织相容性复合体中基因编码的I类或II类糖蛋白，以及独特型α/β链T细胞抗原受体。肽必须与MHC分子结合才能与T细胞抗原受体相互作用，这是T淋巴细胞对抗原识别的MHC限制性的分子基础。在这种三分子相互作用中，肽的氨基酸序列必须指定与两种受体的接触：抗原决定部位残基与MHC受体结合，表位残基与T细胞抗原受体结合。根据已知抗原肽的汇编，已提出两种算法来预测蛋白质中的抗原位点。一种算法使用序列中的线性基序，而另一种算法考虑肽的构象并预测两亲性α螺旋的抗原性。我们在此报告，对抗原位点进行系统的界定精确地确定了一个预测的五肽基序，它是由I类MHC分子呈递并被细胞毒性T淋巴细胞克隆识别的最小抗原决定簇。
