Wang Linghui, Cai Yu, Jing Yihang, Zhu Baoku, Zhu Liping, Xu Youyi
Key Laboratory of Macromolecule Synthesis and Functionalization (MOE), ERC of Membrane and Water Treatment (MOE), Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Key Laboratory of Macromolecule Synthesis and Functionalization (MOE), ERC of Membrane and Water Treatment (MOE), Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
J Colloid Interface Sci. 2014 May 15;422:38-44. doi: 10.1016/j.jcis.2014.02.005. Epub 2014 Feb 13.
Polyethersulfone (PES) membranes with improved hemocompatibility were prepared via solid-liquid interface aminolysis and heparinization. Reactive amino groups were generated by immersing solid PES membranes in proper diamine solution. Heparin was covalent immobilized on the surface via amide bond. The feasibility of surface aminolysis for the introduction of amino groups and the effectiveness for further heparin immobilization were confirmed by surface group analysis. The effect of aminolysis time on surface amino group concentration and bulk mechanical properties was investigated. The surface amino group concentration determined the amount and bioactivity of immobilized heparin chains. SEM images suggested that both the aminolysis and heparinization reaction had little effect on the surface morphology of PES membranes. Contact angle measurement, surface charge analysis, protein and platelet adsorption/adhesion experiment were applied to study the surface properties. The results showed that the heparinized PES membranes displayed enhanced hydrophilicity and hemocompatibility, indicating potential application in blood purification and other blood contacting fields.
通过固液界面氨解和肝素化制备了具有改善血液相容性的聚醚砜（PES）膜。将固态PES膜浸入适当的二胺溶液中以产生反应性氨基。肝素通过酰胺键共价固定在表面上。通过表面基团分析证实了表面氨解引入氨基的可行性以及进一步固定肝素的有效性。研究了氨解时间对表面氨基浓度和本体力学性能的影响。表面氨基浓度决定了固定化肝素链的数量和生物活性。扫描电子显微镜图像表明，氨解和肝素化反应对PES膜的表面形态影响很小。通过接触角测量、表面电荷分析、蛋白质和血小板吸附/粘附实验来研究表面性质。结果表明，肝素化的PES膜表现出增强的亲水性和血液相容性，表明其在血液净化和其他血液接触领域具有潜在应用。
