Palmer Arthur G
Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY 10032, United States.
J Magn Reson. 2014 Apr;241:3-17. doi: 10.1016/j.jmr.2014.01.008.
The perspective reviews quantitative investigations of chemical exchange phenomena in proteins and other biological macromolecules using NMR spectroscopy, particularly relaxation dispersion methods. The emphasis is on techniques and applications that quantify the populations, interconversion kinetics, and structural features of sparsely populated conformational states in equilibrium with a highly populated ground state. Applications to folding, molecular recognition, catalysis, and allostery by proteins and nucleic acids are highlighted.
本文综述了利用核磁共振波谱,特别是弛豫色散方法对蛋白质和其他生物大分子中化学交换现象进行的定量研究。重点是量化与高丰度基态处于平衡状态的稀有构象态的丰度、相互转化动力学和结构特征的技术及应用。文中突出了这些技术在蛋白质和核酸的折叠、分子识别、催化及变构方面的应用。