Kocks J E M, Broekhuijse M L W J
CRV B.V., P.O. Box 454, 6800 AL Arnhem, the Netherlands.
Anim Reprod Sci. 2014 May;146(3-4):98-102. doi: 10.1016/j.anireprosci.2014.02.015. Epub 2014 Mar 2.
Determining the volume of an ejaculate is an important part of processing semen in bovine AI laboratory practice. A multi-AI laboratory study was performed to estimate the density of whole bull semen, and whether the use of this value as a standard is suitable for practical use when semen of different breeds is processed at different AI laboratories. The density of whole bull semen had been determined for 90 ejaculates at five AI laboratories (five breeds). The results showed no effect of bull (p=0.766), breed (p=0.279) and laboratory (p=0.183). All duplicate measurements within the same sample were within the level of agreement (5%). Using the mean value of 1.053g/ml as a golden standard for the density of whole bull semen is therefore suitable for use in routine bovine AI laboratory practice.
确定射精量是牛人工授精实验室操作中精液处理的重要环节。进行了一项多人工授精实验室研究,以估计公牛全精液的密度,以及在不同人工授精实验室处理不同品种精液时,将该值用作标准是否适用于实际应用。在五个人工授精实验室(五个品种)对90份射精样本测定了公牛全精液的密度。结果显示公牛(p = 0.766)、品种(p = 0.279)和实验室(p = 0.183)均无影响。同一样本内的所有重复测量值均在一致水平(5%)内。因此,将1.053g/ml的平均值作为公牛全精液密度的黄金标准适用于牛人工授精实验室的常规操作。