Edström A, Kanje M, Rusovan A
Department of Zoophysiology, University of Lund, Sweden.
Acta Physiol Scand. 1988 Nov;134(3):437-41. doi: 10.1111/j.1748-1716.1988.tb08512.x.
The outgrowth region of the regenerating frog sciatic nerve shows an increased permeability for various drugs. The present experiments indicate that this is also true for vanadate, a substance of special interest in relation to axonal transport. Six days after a bilateral crush lesion, the sciatic nerves, including the spinal ganglia, were incubated in a compartmented chamber. Orthograde transport was assessed from the distribution and the accumulation of [3H]leucine-labelled proteins in the nerve growth region. Retrograde transport was examined by allowing orthogradely transported labelled materials to reverse at the regenerating region and then to accumulate at a ligature during a second incubation period. Vanadate at 0.5 mM and higher concentrations was found to inhibit orthograde and retrograde transport in the outgrowth region (6 mm long at 6 d after crush), whereas 0.2 mM vanadate inhibited transport only in the retrograde direction. The observations lend further support to the idea that the mechanisms of transport in the two directions are different.
再生青蛙坐骨神经的生长区对各种药物的通透性增加。目前的实验表明，钒酸盐也是如此，钒酸盐是一种与轴突运输有关的特别有趣的物质。双侧挤压损伤6天后，将包括脊髓神经节在内的坐骨神经在分隔小室中孵育。通过[3H]亮氨酸标记蛋白在神经生长区的分布和积累来评估顺行运输。通过让顺行运输的标记物质在再生区逆转，然后在第二次孵育期间在结扎处积累来检查逆行运输。发现0.5 mM及更高浓度的钒酸盐会抑制生长区（挤压后6天时长6 mm）的顺行和逆行运输，而0.2 mM钒酸盐仅抑制逆行方向的运输。这些观察结果进一步支持了两个方向的运输机制不同这一观点。
