采用酶免疫测定法检测登革病毒NS1抗原。

Detection of the dengue virus NS1 antigen using an enzyme immunoassay.

作者信息

Anderson Neil W, Jespersen Deborah J, Rollins Leonard, Seaton Brent, Prince Harry E, Theel Elitza S

机构信息

Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.

Focus Diagnostics, Cypress, CA 90630, USA.

出版信息

Diagn Microbiol Infect Dis. 2014 Jun;79(2):194-7. doi: 10.1016/j.diagmicrobio.2014.02.001. Epub 2014 Feb 22.

DOI:10.1016/j.diagmicrobio.2014.02.001

PMID:24657172

Abstract

Current diagnostic methods for dengue virus (DV) rely primarily on detection of anti-DV antibodies and/or DV RNA by reverse transcriptase (RT) PCR. Several limitations exist however: seroconversion is delayed following infection, and DV RT-PCR assays are not yet readily available. The DV nonstructural protein 1 (NS1) antigen is an alternative acute phase DV biomarker, and here, we evaluated the new InBios (InBios International, Inc., Seattle, WA, USA) DENV Detect(TM) NS1 enzyme-linked immunoassay (ELISA) compared to DV RT-PCR and serology for detection of recent DV infection. We report a positive, negative, and overall percent agreement of 96% (24/25), 86.0% (43/50), and 89.3% (67/75) for the InBios NS1 ELISA compared to DV RT-PCR. Performance of the NS1 ELISA compared to serology for anti-DV IgM antibodies showed a positive, negative, and overall percent agreement of 78.0% (85/109), 90.7% (333/367), and 87.8% (418/476), respectively. Collectively, the InBios NS1 ELISA can be used as an alternative to DV RT-PCR for identification of acute DV infection.

摘要

目前登革病毒（DV）的诊断方法主要依赖于通过逆转录酶（RT）PCR检测抗DV抗体和/或DV RNA。然而，存在一些局限性：感染后血清转化延迟，并且DV RT-PCR检测方法尚不易获得。DV非结构蛋白1（NS1）抗原是一种替代的急性期DV生物标志物，在此，我们评估了新型的InBios（美国华盛顿州西雅图市InBios国际公司）DENV Detect™ NS1酶联免疫吸附测定（ELISA）与DV RT-PCR和血清学方法相比用于检测近期DV感染的情况。与DV RT-PCR相比，我们报告InBios NS1 ELISA的阳性、阴性和总体一致率分别为96%（24/25）、86.0%（43/50）和89.3%（67/75）。与抗DV IgM抗体血清学相比，NS1 ELISA的表现分别为阳性一致率78.0%（85/109）、阴性一致率90.7%（333/367）和总体一致率87.8%（418/476）。总体而言，InBios NS1 ELISA可作为DV RT-PCR的替代方法用于识别急性DV感染。