Granger D, Leo Y S, Lee L K, Theel E S
Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.
Institute of Infectious Diseases and Epidemiology, Communicable Disease Centre, Tan Tock Seng Hospital, Singapore.
Diagn Microbiol Infect Dis. 2017 Jun;88(2):120-124. doi: 10.1016/j.diagmicrobio.2017.03.015. Epub 2017 Mar 30.
Accuracy of the InBios DENV Detect IgM, IgG and NS1 antigen (Ag) ELISAs (Seattle, WA) for detection of dengue virus (DENV) infection were evaluated using 100 retrospectively selected sera from acutely febrile patients presenting to a Singapore hospital. The InBios DENV NS1, IgM and IgG ELISAs had an overall sensitivity of 83.6%, 40% and 58.2% and an overall specificity of 97.8%, 97.8% and 55.6%, respectively. Simultaneous testing for NS1 and IgM-antibodies yielded a sensitivity and specificity of 85.5% and 95.5%, respectively, which did not significantly differ from testing for NS1 Ag alone. Using sera positive for IgM- or IgG-class antibodies to six common arboviruses, the InBios IgM and IgG ELISAs showed an overall analytic specificity of 89.2% and 66.4%, respectively. This study suggests that recent DENV infection can reliably be detected by the InBios NS1 Ag ELISA alone and that InBios DENV IgG reactivity should be interpreted with caution.
使用从新加坡一家医院就诊的急性发热患者中回顾性选择的100份血清，评估了InBios登革病毒（DENV）检测IgM、IgG和NS1抗原（Ag）酶联免疫吸附测定法（ELISAs）（华盛顿州西雅图）检测登革病毒（DENV）感染的准确性。InBios DENV NS1、IgM和IgG酶联免疫吸附测定法的总体敏感性分别为83.6%、40%和58.2%，总体特异性分别为97.8%、97.8%和55.6%。同时检测NS1和IgM抗体的敏感性和特异性分别为85.5%和95.5%，与单独检测NS1抗原无显著差异。使用对六种常见虫媒病毒呈IgM或IgG类抗体阳性的血清，InBios IgM和IgG酶联免疫吸附测定法的总体分析特异性分别为89.2%和66.4%。这项研究表明，仅通过InBios NS1抗原酶联免疫吸附测定法就能可靠地检测近期的DENV感染，并且InBios DENV IgG反应性的解读应谨慎。
