Chakrabarti Rina, Srivastava Praveen Kumar, Verma Nandini, Sharma JaiGopal
Aqua Research Lab, Department of Zoology, University of Delhi, Delhi 110007, India.
Aqua Research Lab, Department of Zoology, University of Delhi, Delhi 110007, India.
Fish Shellfish Immunol. 2014 Nov;41(1):64-9. doi: 10.1016/j.fsi.2014.03.007. Epub 2014 Mar 20.
Evaluation of functional mechanism of immunostimulant in fish is essential for realizing their therapeutic potential. The impact of dietary supplementation of seeds of Achyranthes aspera on the immune system of Catla catla was studied. Catla (37.73 ± 6.7 g) were fed with test diets containing 0.5 (D1) and 1.0% (D2) seeds of A. aspera and control diet (D3) for 50 days. Then fish were immunized with heat-killed Aeromonas hydrophila. Blood and tissue samples were collected after 7 days of immunization. Three fish were sacrificed for each parameter. Respiratory burst activity, bactericidal activity and antibody response were significantly (P < 0.05) higher in test diets fed catla compared to the control one. Nitric oxide synthase and serum lysozyme were significantly (P < 0.05) higher in fish fed with diet D2 compared to the other treatments. There were up-regulations of TNFα, lysozyme c and g gene expressions in kidney and IL-10, lysozyme c and g expressions in hepatopancreas of test diets fed fish compared to the control one. The expression of IL-10 was down-regulated in the kidney of D2 diet fed fish compared to others. In the gill, significantly (P < 0.05) higher expressions of TNFα was found in D1 diet feed fish and lysozyme c and in D2 diet fed fish compared to the other groups. This study showed tissue-specific gene expression pattern in catla. An inverse relationship was found between the expression of TNFα and IL-10 in kidney of catla.
评估免疫刺激剂在鱼类中的作用机制对于实现其治疗潜力至关重要。本研究探讨了饲料中添加牛膝种子对印度鲮免疫系统的影响。将体重为(37.73 ± 6.7 g)的印度鲮分别投喂含0.5%（D1）和1.0%（D2）牛膝种子的试验饲料以及对照饲料（D3），持续50天。之后用热灭活的嗜水气单胞菌对鱼进行免疫。免疫7天后采集血液和组织样本。每个参数取3条鱼进行分析。与对照饲料组相比，投喂试验饲料的印度鲮的呼吸爆发活性、杀菌活性和抗体反应显著（P < 0.05）升高。与其他处理组相比，投喂D2饲料的鱼的一氧化氮合酶和血清溶菌酶显著（P < 0.05）升高。与对照饲料组相比，投喂试验饲料的鱼的肾脏中TNFα、溶菌酶c和g基因表达上调，肝胰腺中IL-10、溶菌酶c和g表达上调。与其他组相比，投喂D2饲料的鱼的肾脏中IL-10表达下调。在鳃中，与其他组相比，投喂D1饲料的鱼和投喂D2饲料的鱼的TNFα、溶菌酶c表达显著（P < 0.05）升高。本研究显示了印度鲮组织特异性基因表达模式。在印度鲮的肾脏中发现TNFα和IL-10的表达呈负相关。
