Kumar Neelesh, Sharma JaiGopal, Singh Samar Pal, Singh Amarjeet, Hari Krishna V, Chakrabarti Rina
Department of Biotechnology, Delhi Technological University, Bawana Road, Delhi 110 042, India.
Aqua Research Lab, Department of Zoology, University of Delhi, Delhi 110 007, India.
Heliyon. 2019 Feb 18;5(2):e01246. doi: 10.1016/j.heliyon.2019.e01246. eCollection 2019 Feb.
The immunostimulatory and disease resistance properties of herb L. (Amaranthaceae) were evaluated in rohu in pond. Rohu fry (1.9 ± 0.08 g) were cultured in hapas (25 hapa) set inside a pond and were fed with two experimental diets containing 0.5% seeds (D1) and leaves (D2) of and control diet (D3). Fish were challenged with after 80 days. The cumulative mortality rate of fish was significantly ( < 0.05) higher in D3 (28-48%) compared to others. Average weight was significantly ( < 0.05) higher in D1 (6.5-12.5%) compared to other treatments. Myeloperoxidase and nitric oxide synthase levels were significantly ( < 0.05) higher in D1 and D2 compared to D3. Thiobarbituric acid reactive substances and carbonyl protein levels were significantly ( < 0.05) lower in hepatopancreas and kidney of D1 compared to others. In hepatopancreas, the expressions of lysozyme C, loysozyme G, TNF-α, IL-10 and IL-1β were significantly ( < 0.05) higher in D1 compared to others. This treatment was followed by D2. In kidney, lysozyme G and TNF-α levels were significantly ( < 0.05) higher in D1 and D2 compared to D3. Whereas, IL-10 and IL-1β were significantly ( < 0.05) down-regulated and up-regulated, respectively in kidney of D2. There was up-regulation ( < 0.05) of TLR-4 in hepatopancreas and kidney of D1 and D2 diets fed rohu, respectively compared to others.
对蓼科草本植物L.的免疫刺激和抗病特性在池塘养殖的印度魮中进行了评估。将印度魮鱼苗（1.9±0.08克）养殖在池塘内设置的网箱（25个网箱）中，并用两种实验饲料进行投喂，其中一种饲料含有0.5%的该植物种子（D1），另一种含有0.5%的该植物叶子（D2），还有对照饲料（D3）。80天后，用[具体病原体名称未给出]对鱼进行攻毒。与其他组相比，D3组鱼的累积死亡率显著更高（P<0.05）（28 - 48%）。与其他处理组相比，D1组鱼的平均体重显著更高（P<0.05）（6.5 - 12.5%）。与D3组相比，D1组和D2组的髓过氧化物酶和一氧化氮合酶水平显著更高（P<0.05）。与其他组相比，D1组鱼肝胰腺和肾脏中的硫代巴比妥酸反应性物质和羰基蛋白水平显著更低（P<0.05）。在肝胰腺中，与其他组相比，D1组溶菌酶C、溶菌酶G、肿瘤坏死因子-α、白细胞介素-10和白细胞介素-1β的表达显著更高（P<0.05）。其次是D2组。在肾脏中，与D3组相比，D1组和D2组的溶菌酶G和肿瘤坏死因子-α水平显著更高（P<0.05）。而在D2组鱼的肾脏中，白细胞介素-10显著下调（P<0.05），白细胞介素-1β显著上调（P<0.05）。与其他组相比，分别投喂D1和D2饲料的印度魮的肝胰腺和肾脏中Toll样受体4（TLR-4）上调（P<0.05）。
