Korsun N S, Nosik D N, Novokhatskiĭ A S, Trushinskaia G N
Antibiot Khimioter. 1988 Nov;33(11):827-30.
Preparation of highly active rabbit antisera (AS) to human recombinant alpha 2-interferon and their use for studying biological properties of natural and plasmid alpha-interferons are described. By exhaustion of AS by alpha 3-interferon there were prepared practically monospecific AS not reacting with antigenic determinants of alpha 3-interferon. It was found that alpha 3-interferon represented a significant portion of human lymphoblastoid interferons and was included in PH-labile alpha-interferon from serum of patients with Kaposi carcinoma. AS to alpha 2-interferon completely neutralized antiviral and antiproliferative activity of the homologous subtype alpha-interferon and stimulation of cytotoxicity of human natural killer cells induced by it. It neutralized also the same effects of the heterologous subtypes (alpha 3 and alpha F/D) and leukocytic interferon, but the neutralization level was lower. The results of the study confirmed the polyfunctional nature of the interferon molecule.
描述了针对人重组α2干扰素制备高活性兔抗血清（AS）及其用于研究天然和质粒α干扰素生物学特性的情况。通过用α3干扰素耗尽AS，制备出了实际上不与α3干扰素抗原决定簇反应的单特异性AS。发现α3干扰素占人淋巴母细胞干扰素的很大一部分，并包含在卡波西肉瘤患者血清中对酸不稳定的α干扰素中。针对α2干扰素的AS完全中和了同源亚型α干扰素的抗病毒和抗增殖活性以及由其诱导的人自然杀伤细胞细胞毒性的刺激作用。它也中和了异源亚型（α3和αF/D）和白细胞干扰素的相同作用，但中和水平较低。研究结果证实了干扰素分子的多功能性质。
