Wahab Rizwan, Siddiqui Maqsood A, Saquib Quaiser, Dwivedi Sourabh, Ahmad Javed, Musarrat Javed, Al-Khedhairy Abdulaziz A, Shin Hyung-Shik
A.R. Al-Jeraisy Chair for DNA Research, College of Science, Department of Zoology, King Saud University, Riyadh 11451, Saudi Arabia.
A.R. Al-Jeraisy Chair for DNA Research, College of Science, Department of Zoology, King Saud University, Riyadh 11451, Saudi Arabia.
Colloids Surf B Biointerfaces. 2014 May 1;117:267-76. doi: 10.1016/j.colsurfb.2014.02.038. Epub 2014 Mar 2.
Liver and breast cancer are the most traumatic diseases because they affect the major organs of the body. Nanomedicine recently emerged as a better option for the treatment of these deadly diseases. As a result, many nanoparticles have been used to treat cancer cell lines. Of the various nanoparticles, zinc oxide exhibits biocompatibility. Therefore, the aim of the present study was to investigate the activity of zinc oxide nanoparticles (ZnO-NPs) against HepG2 and MCF-7 cells. The NPs (∼13±2 nm) were prepared via a non-protonated chemical route and were well-characterized through standard techniques. The study showed that treatment with NPs is notably effective against the proliferation of HepG2 and MCF-7 cancer cells in a dose-dependent manner. The MTT (3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide, a tetrazole) assays revealed the concentration-dependent cytotoxic effects of NPs in range of 2.5-100 μg/ml. HepG2 and MCF-7 cells were exposed to ZnO-NPs and exhibited a significant reduction in their cell viability (95% and 96%; p<0.05) in response to a very low concentration (25 μg/ml) of the ZnO-NPs; this finding was confirmed with FACS (fluorescence-activated cell sorting) data. The reduction in cell viability in response to NP treatment induces cytotoxicity in the cultured cells. The quantitative RT-PCR (real-time polymerase chain reaction) results demonstrate that the exposure of HepG2 cells to ZnO-NPs results in significant upregulation of the mRNA expression level of Bax, p53, and caspase-3 and the down regulation of the anti-apoptotic gene Bcl-2. The NPs were also tested against five pathogenic bacteria through the disk diffusion method, and their antibacterial activities were compared with that of ZnO salt.
肝癌和乳腺癌是最具创伤性的疾病，因为它们会影响身体的主要器官。纳米医学最近成为治疗这些致命疾病的更好选择。因此，许多纳米颗粒已被用于治疗癌细胞系。在各种纳米颗粒中，氧化锌具有生物相容性。因此，本研究的目的是研究氧化锌纳米颗粒（ZnO-NPs）对HepG2和MCF-7细胞的活性。通过非质子化化学路线制备了纳米颗粒（约13±2纳米），并通过标准技术对其进行了充分表征。研究表明，纳米颗粒处理对HepG2和MCF-7癌细胞的增殖具有显著的剂量依赖性抑制作用。MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐，一种四氮唑）测定显示纳米颗粒在2.5-100μg/ml范围内具有浓度依赖性细胞毒性作用。将HepG2和MCF-7细胞暴露于ZnO-NPs中，在极低浓度（25μg/ml）的ZnO-NPs作用下，其细胞活力显著降低（分别为95%和96%；p<0.05）；这一发现得到了FACS（荧光激活细胞分选）数据的证实。纳米颗粒处理导致的细胞活力降低在培养细胞中诱导了细胞毒性。定量RT-PCR（实时聚合酶链反应）结果表明，HepG2细胞暴露于ZnO-NPs后，Bax、p53和caspase-3的mRNA表达水平显著上调，抗凋亡基因Bcl-2的表达水平下调。还通过纸片扩散法对纳米颗粒进行了针对五种病原菌的测试，并将其抗菌活性与氧化锌盐的抗菌活性进行了比较。
